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Dinoflagellate Tripos species composition and seasonal dynamics in Jiaozhou Bay revealed through 18S rDNA V4-based metabarcoding analysis

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Abstract

Tripos is one of the largest dinoflagellate genera with ~800 reported taxa widely distributed in global marine ecosystems. These diverse Tripos species are ecologically important as critical primary producers and some Tripos species are also harmful algal bloom (HAB) species that may pose significant negative impact on marine ecosystems. However, due to the high morphological intraspecific variability and plasticity of numerous Tripos species, morphology-based identification and tracking of their dynamic changes have been both technically challenging and inconclusive. In this project, we carried out metabarcoding analysis of Tripos species in Jiaozhou Bay (JZB) through amplifying and sequencing of the common molecular marker 18S rDNA V4. The analysis identified five Tripos species (T. furca, T. fusus, T. eugrammus, T. falcatus and T. massiliensis), including two (T. eugrammus and T. falcatus) identified for the first time in JZB, demonstrating the competitive strength of metabarcoding analysis. This analysis of time-series samples collected in JZB revealed interesting seasonal dynamic changes of Tripos species, with different species demonstrating differential seasonal preferences. This analysis also suggested the possible existence of 11 additional Tripos species in JZB. The inconclusive identification of these 11 potential Tripos species indicated that the resolution of the common molecular marker 18S rDNA V4 applied in this study is inadequate and that the molecular reference data for Tripos is still limited. Selection of molecular markers with higher resolution (such as 28S rDNA D1-6) and the completion of reference sequences of Tripos species may facilitate more accurate identification of and tracking of their dynamic changes.

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Data availability

The sequencing results (raw data) have been submitted to NCBI, and the BioProject numbers are PRJNA577777 and PRJNA733859.

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Acknowledgements

We are grateful to colleagues from the Jiaozhou Bay Marine Ecosystem Research Station for the opportunity to participate in the investigation expeditions. Statistical analyses were supported by Oceanographic Data Center, IOCAS.

Funding

This research was supported by the Natural Science Foundation of China (42176162), the National Key Research and Development (R&D) Program of China (grant No.2022YFC3105200), the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (LSKJ202203700), the Strategic Priority Research Program of Chinese Academy of Sciences (XDB42000000), the Chinese Academy of Sciences Pioneer Hundred Talents Program (to Nansheng Chen), the Taishan Scholar Project Special Fund (to Nansheng Chen).

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  1. Xianliang Huang and Kuiyan Liu are equal contribution.

Authors and Affiliations

  1. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Xianliang Huang, Kuiyan Liu, Xiangxiang Ding, Shuya Liu, Zhongmei Cui & Nansheng Chen

  2. Laboratory of Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, 266200, China

    Xianliang Huang, Kuiyan Liu, Xiangxiang Ding, Shuya Liu, Zhongmei Cui, Yongfang Zhao & Nansheng Chen

  3. College of Marine Science, University of Chinese Academy of Sciences, Beijing, 100039, China

    Xianliang Huang, Kuiyan Liu & Xiangxiang Ding

  4. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China

    Xianliang Huang, Kuiyan Liu, Xiangxiang Ding, Shuya Liu, Zhongmei Cui, Yongfang Zhao & Nansheng Chen

  5. Institute of Oceanology, Jiaozhou Bay National Marine Ecosystem Research Station, Chinese Academy of Sciences, Qingdao, 266071, China

    Yongfang Zhao

  6. Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, V5A 1S6, Canada

    Nansheng Chen

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Contributions

Nansheng Chen contributed to the study conception and design. XianliangHuang, Kuiyan Liu performed metabarcoding analyses. Shuya Liu, Zongmei Cui, Xiangxiang Ding and Yongfang Zhao carried out sample collection. Xianliang Huang wrote the first draft of the manuscript. All authors read and approved the final manuscript.

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Huang, X., Liu, K., Ding, X. et al. Dinoflagellate Tripos species composition and seasonal dynamics in Jiaozhou Bay revealed through 18S rDNA V4-based metabarcoding analysis. J Appl Phycol (2024). https://doi.org/10.1007/s10811-024-03239-5

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