Abstract
The extracellular matrix (ECM), the main component of the extracellular space, mediates signal transmission between cells and controls their key functions: proliferation, differentiation, and migration. The relevance of studying ECM research is stipulated to the wide range of its biological properties, which can be used in regenerative medicine and bioengineering. Particular interest is presented the study of the regulatory activity on various cellular functions of cell-derived decellularized ECM (dECM). In this work, we tested the hypothesis about the modulating effect of dECM deposited by young MSC from Wharton’s jelly on the aging phenotype of endometrial human multipotent mesenchymal stromal cells (eMSCs), which the cells acquired in response to oxidative stress. This aspect of ECM functioning in the context of eMSCs has not yet been considered. A comparative study of H2O2-induced senescence of eMSCs cultured on dECM and on plastic for a long time showed a significant change in the hallmarks of aging in the cell population maintained on dECM. Taken together, the results obtained suggest that the dECM is able to partially reverse (retard) premature senescence of eMSCs in response to oxidative stress, as well as expanding the understanding of the ECM as a regulator of the functional activity of cells.
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ACKNOWLEDGMENTS
MSCWJ-1 cells were obtained from the “Collection of Vertebrate Cell Cultures” Center for Collective Use supported by the Ministry of Education and Science of the Russian Federation, no. 075-15-2021-683.
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The work was supported by an Internal grant from the Institute of Cytology, Russian Academy of Sciences, in 2023.
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Abbreviations: AF—autofluorescence; ROS—reactive oxygen species; dECM—decellularized extracellular matrix; CGM—complete growth medium; MSC and eMSC—human multipotent mesenchymal stromal cell and endometrial MSC, respectively; PBS—phosphate-buffered saline solution; SA-β-Gal—senescence associated β-galactosidase.
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Burova, E.B., Perevoznikov, I.E. & Ushakov, R.E. Decellularized Extracellular Matrix Slows Down Premature Senescence of Human Endometrial Mesenchymal Stromal Cells. Cell Tiss. Biol. 18, 173–182 (2024). https://doi.org/10.1134/S1990519X23700037
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DOI: https://doi.org/10.1134/S1990519X23700037