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Design and Synthesis of an Integrated Nanozyme by Immobilizing Glucose Oxidase on an Iron-Based Metal-Organic Framework for the Development of a Glucose Assay

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Abstract

Considering the application prospects of a cascade catalytic system in biomedical analysis, it remains significant to develop a cascade catalytic system for one-step rapid colorimetric detection. Herein, PVP/NH2-MIL-101(Fe) possessing enhanced peroxidase (POD)-like activity was successfully synthesized by microwave-assisted and polyvinylpyrrolidone (PVP) modification strategies. Then, PVP/NH2-MIL-101(Fe) and glucose oxidase (GOx) were covalently coupled via the bifunctional cross-linker glutaraldehyde, the GOx@PVP/NH2-MIL-101(Fe) with dual enzymatic functional activities (POD-like and GOx activities) was subsequently synthesized. GOx@PVP/NH2-MIL-101(Fe) had strong affinities for the substrates glucose, 3,3',5,5'-tetramethylbenzidine, and hydrogen peroxide. Based on this specific nanozyme GOx@PVP/NH2-MIL-101(Fe), a one-step colorimetric assay for rapid glucose determination was built. This detection method not only took 20 min to analyze but also possessed a linear range of 10–250 µM with a detection limit of 1.05 µM. Furthermore, the cascade catalytic system was applied to determine glucose concentrations in human serum samples. Compared with commercial blood glucose meters, the proposed method was also accurate and effective, making it a candidate method for determining blood glucose.

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Funding

This work was financially supported by the Natural Science Foundation of the Anhui Higher Education Institutions of China (No. 2023AH051555).

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Authors and Affiliations

  1. School of Chemistry and Materials Engineering, Anhui Key Laboratory of Low Temperature Co-fired Materials, Huainan Normal University, 232038, Huainan, Anhui Province, P.R. China

    Yanyan Xing

  2. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 117004, Shenyang, Liaoning Province, P.R. China

    Yanyan Xing, Sijia Zhang & Xiaohong Hou

  3. School of Pharmacy, Shenyang Pharmaceutical University, 117004, Shenyang, Liaoning Province, P.R. China

    Meiling Chen & Huining Lan

  4. School of Science, Tianjin University, 300072, Tianjin, P.R. China

    Jiabi Xu

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  1. Yanyan Xing
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Correspondence to Xiaohong Hou.

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Xing, Y., Chen, M., Lan, H. et al. Design and Synthesis of an Integrated Nanozyme by Immobilizing Glucose Oxidase on an Iron-Based Metal-Organic Framework for the Development of a Glucose Assay. J Anal Chem 79, 400–411 (2024). https://doi.org/10.1134/S1061934824040142

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