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Molecular epidemiology and resistance mechanisms of tigecycline-non-susceptible Acinetobacter baumannii isolated from a tertiary care hospital in Chongqing, China

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Abstract

We studied 34 isolates of Tigecycline-Non-Susceptible A. baumannii (TNAB) obtained from clinical specimens at a large tertiary care hospital in Chongqing, China. These 34 strains belonged to 8 different clones including ST195 (35.3%) and ST208 (17.7%). EBURST analysis found that these 8 ST types belonged to the Clonal Complex 92. Tigecycline resistance-associated genes adeR, adeS, adeL, adeN, rrf, rpsJ, and trm were detected in most strains. The expression level of the resistance-nodulation-cell division (RND) efflux pumps in TNAB strains was higher than the reference strain ATCC19606. 58.8% of strains had a decrease in the tigecycline minimum inhibitory concentration (MIC) after the addition of carbonyl cyanide 3-chlorophenylhydrazone (CCCP). The TNAB strains in our hospital have a high degree of affinity and antibiotic resistance. Regular surveillance should be conducted to prevent outbreaks of TNAB epidemics.

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Data availability

The datasets in the current study are available from the corresponding author upon reasonable request.

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Funding

This study was supported by the National Natural Science Foundation of China (81572055).

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Authors and Affiliations

  1. Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, People’s Republic of China

    Jiajia Liao, Jinzhu Huang, Peiwen Xia, Bingxue Yang, Zijun Dang, Shengli Luo, Shiyu Tang, Yuqiong Li, Qi Han & Yun Xia

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  1. Jiajia Liao
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  2. Jinzhu Huang
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  3. Peiwen Xia
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  4. Bingxue Yang
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  5. Zijun Dang
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  6. Shengli Luo
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  7. Shiyu Tang
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  8. Yuqiong Li
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  9. Qi Han
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  10. Yun Xia
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Contributions

Yun Xia and Jiajia Liao contributed to the study conception and design. Material preparation, data collection and were performed by Shengli Luo, Shiyu Tang, Yuqiong Li, and Qi Han. Data analysis and curation were conducted by Jinzhu Huang, Peiwen Xia, Bingxue Yang, and Zijun Dang. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Yun Xia.

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All of the authors declare no conflict of interest in this study.

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The study was conducted in accordance with the Declaration of Helsinki and approved by the medical center’s ethical committee of the First Affiliated Hospital of Chongqing Medical University.

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Liao, J., Huang, J., Xia, P. et al. Molecular epidemiology and resistance mechanisms of tigecycline-non-susceptible Acinetobacter baumannii isolated from a tertiary care hospital in Chongqing, China. Eur J Clin Microbiol Infect Dis (2024). https://doi.org/10.1007/s10096-024-04832-9

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