From the journal:

Chemical Communications

Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA†

Cui-Lian Lin,ab   Wen-Da Chen,ab   Li Liu ORCID logo ab  and  Liang Cheng ORCID logo *ab  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
E-mail: chengl@iccas.ac.cn

b University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

Through the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events.

Graphical abstract: Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

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Article information

DOI
https://doi.org/10.1039/D4CC01106F
Article type
Communication
Submitted
08 Mar 2024
Accepted
17 Apr 2024
First published
18 Apr 2024

Chem. Commun., 2024, Advance Article

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Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

C. Lin, W. Chen, L. Liu and L. Cheng, Chem. Commun., 2024, Advance Article , DOI: 10.1039/D4CC01106F

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