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Dietary Poly-β-Hydroxybutyrate Improved the Growth, Non-specific Immunity, Digestive Enzyme Activity, Intestinal Morphology, Phagocytic Activity, and Disease Resistance Against Vibrio parahaemolyticus of Pacific White Shrimp, Penaeus vannamei

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Abstract

This study assessed the effects of dietary supplementation of poly-β-hydroxybutyrate (PHB) on growth performance, feed efficiency, non-specific immunity, digestive enzyme capacity, phagocytic activity, hemocyte count, intestinal morphology, and disease resistance against Vibrio parahaemolyticus of Pacific white shrimp (Penaeus vannamei). Six diets were prepared by supplementing graded levels of PHB at 0.00, 0.25, 0.50, 1.00, 2.00, and 4.00% (Con, P0.25, P0.5, P1.0, P2.0, and P4.0, respectively). Triplicate groups of 90 shrimps (initial body weight 0.25 ± 0.01 g) per treatment were randomly assigned and fed an experimental diet for 56 days. The growth performance of shrimp was significantly improved by 1% dietary PHB supplementation. PHB-included diets fed shrimp showed significantly improved hepatopancreatic trypsin, chymotrypsin, and pepsin activities. Villus height was significantly increased with dietary PHB supplementation, and villus width was increased at a 1% inclusion level. P0.25, P0.5, and P4.0 groups significantly increased phenoloxidase activity, and the P2.0 group significantly increased anti-protease activity compared to the Con group. The survival of shrimp challenged against V. parahaemolyticus was higher in P0.5, P1.0, and P2.0 groups than in the Con diet. Dietary PHB supplementation improved weight gain, digestive enzyme activity, intestinal morphology, non-specific immunity, and disease resistance against V. parahaemolyticus of shrimp. According to the above observations, the optimal dietary PHB supplementation level for maximum weight gain would be 1% for Pacific white shrimp.

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Data Availability

All the datasets analyzed in this study are available from the corresponding author upon reasonable request.

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Funding

This study was funded by CJ CheilJedang Co., Ltd. and Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2019R1A6A1A03033553).

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Authors and Affiliations

  1. Department of Marine Life Sciences, Jeju National University, Jeju, 63243, South Korea

    Suhyeok Kim, Jaebeom Shin, Nalin Medagoda & Kyeong-Jun Lee

  2. Protein Solution Department, CJ Cheiljedang BIO, Seoul, 04560, South Korea

    Sera Choi

  3. R&D, Cheiljedang White BIO, CJ, Suwon, 16495, South Korea

    So Yun Park & Jeung-Yil Park

  4. Marine Science Institute, Jeju National University, Jeju, 63333, South Korea

    Kyeong-Jun Lee

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  1. Suhyeok Kim
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Contributions

Suhyeok Kim participated in the feeding trial, sampling, analyses, and manuscript preparation. Jaebeom Shin and Nalin Medagoda edited the manuscript and analyzed the parameters. Se Ra Choi, So Yun Park, and Jeung-Yil Park designed the experiment, reviewed and edited the manuscript, and provided ingredients. Kyeong-Jun Lee organized and supervised the whole study and completed the manuscript.

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Correspondence to Kyeong-Jun Lee.

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The protocols of this feeding trial were evaluated and approved by the Institutional Animal Care and Use Committee of Jeju National University.

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Kim, S., Shin, J., Medagoda, N. et al. Dietary Poly-β-Hydroxybutyrate Improved the Growth, Non-specific Immunity, Digestive Enzyme Activity, Intestinal Morphology, Phagocytic Activity, and Disease Resistance Against Vibrio parahaemolyticus of Pacific White Shrimp, Penaeus vannamei. Mar Biotechnol (2024). https://doi.org/10.1007/s10126-024-10317-9

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