Abstract
Pancreatic cancer is difficult to manage owing to the challenges involved in its treatment and nursing. This study aimed to clarify the roles and mechanisms of action of Poly (A)-binding protein cytoplasmic 1 (PABPC1) on pancreatic cancer. The expression of PABPC1 in pancreatic cancer tissues and cell lines was detected using RT-qPCR and western blotting. The effects of PABPC1 on proliferation, apoptosis, epithelial-mesenchymal transition (EMT), and the PI3K/AKT signaling pathway in pancreatic cancer cells were further investigated using MTT assays, flow cytometry, and western blotting. The expression of PABPC1 was significantly upregulated in pancreatic cancer tissues and cells, whereas PABPC1 downregulation inhibited pancreatic cancer cell proliferation, induced apoptosis, decreased the expression of EMT-associated proteins, and exerted a regulatory effect by inhibiting the PI3K/AKT signaling pathway. In addition, the findings indicated that PABPC1 over-expression significantly promoted pancreatic cancer cell proliferation, inhibited apoptosis, decreased the expression of E-cadherin, enhanced N-cadherin expression, and activating the PI3K/AKT signaling pathway. PABPC1 silencing significantly inhibited proliferation and EMT and induced apoptosis in pancreatic cancer cells. These findings provide novel insights into the role of PABPC1 in the development of pancreatic cancer.
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The datasets used and/or analyzed in the current study are available from the corresponding author upon reasonable request.
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Changren Zhu and Cuimei Wang contributed to data collection, statistical analysis, data interpretation and manuscript preparation. Jun Zheng contributed to data collection and manuscript preparation. Xiaodong Wang, Shuangshuang Dong, and Qing Xu contributed to data collection and data interpretation. All authors have read and approved the final manuscript.
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Supplementary material 1 (TIF 1380.6 kb)
Effect of PABPC1-siRNA on the proliferation and apoptosis of normal human pancreatic epithelial cell line HPDE 6-C7.HPDE 6-C7 cells were transfected with control-siRNA of PABPC1-siRNA for 48 h. A Cell proliferation was assessed by MTT assay. B and C Cell apoptosis was determined by FCM
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Zhu, C., Wang, C., Wang, X. et al. PABPC1 silencing inhibits pancreatic cancer cell proliferation and EMT, and induces apoptosis via PI3K/AKT pathway. Cytotechnology (2024). https://doi.org/10.1007/s10616-024-00626-1
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DOI: https://doi.org/10.1007/s10616-024-00626-1