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Characterization of Bacterial Isolates from Tailings Pond and Their Resistance to Heavy Metals and Antibiotics

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Abstract

lWater samples were collected from a tailings pond wastewater site in the Rudnik mountain area, where polymetallic ore flotation processes generate high concentrations of Pb2+, Zn2+, and Cu2+. The study aimed to identify microorganisms resistant to heavy metals and assess their potential for bioremediation. Growth capabilities under varying conditions, including temperature, pH, and NaCl concentrations, were analyzed using a spectrophotometer. Minimal inhibitory and lethal concentrations of tested substances were determined for both planktonic cells and their biofilms. Key isolates, namely Bacillus altitudinis PMFKG-R3, B. pumilus PMFKG-R15, B. cereus PMFKG-R46, Pseudomonas veronii PMFKG-R30, and Pantoea agglomerans PMFKG-R20, demonstrated growth ability at both 22 and 37°C and exhibited halotolerance, albeit sensitivity to acidic pH. Most isolates in both planktonic and biofilm forms displayed notable resistance to heavy metals, particularly Pb2+ and Zn2+, in line with the sampling location. Notably, planktonic cells were sensitive to antibiotics, while biofilms exhibited slightly higher resistance. Promising candidates for bioremediation purposes were identified in P. veronii PMFKG-R30 and P. agglomerans PMFKG-R20, which displayed resistance to heavy metals and sensitivity to antibiotics.

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Funding

This work was supported by the Serbian Ministry of Education, Science and Technological Development (Agreement no. 451-03-68/2022-14/200122), Multilateral scientific and technological cooperation in the Danube region for 2020-2022. year (DS10) 337-00-00322/2019-09/107 Metal microorganism’s interaction as a basis for progressive biotechnological processes and COST Action 18113 (STSM grant ECOSTSTSM-Request-CA18113-45768) EuroMicropH—Understanding and exploiting the impacts of low pH on micro-organisms.

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Authors and Affiliations

  1. University of Kragujevac, Faculty of Science, Department of Biology and Ecology, 34000, Kragujevac, Republic of Serbia

    I. D. Radojević, K. G. Ćirković & A. M. Ostojić

  2. University of Kragujevac, Institute for Information Technologies, Department of Science, 34000, Kragujevac, Republic of Serbia

    M. Ž. Grujović & K. G. Mladenović

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  1. I. D. Radojević
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  2. K. G. Ćirković
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  3. M. Ž. Grujović
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  4. K. G. Mladenović
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Correspondence to K. G. Ćirković.

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Supplementary Information

10438_2024_8712_MOESM1_ESM.pdf

Fig. S1 . MALDI-TOF MS spectra of isolate B. altitudinis PMFKG-R3; the absolute intensities of the ions are shown on the y axis, and the masses (m/z) of the ions are shown on the x-axis.

Fig. S2 . MALDI-TOF MS spectra of isolate B. pumilus PMFKG-R15; the absolute intensities of the ions are shown on the y axis, and the masses (m/z) of the ions are shown on the x-axis.

Fig. S3 . MALDI-TOF MS spectra of isolate B. cereus PMFKG-R46; the absolute intensities of the ions are shown on the y axis, and the masses (m/z) of the ions are shown on the x-axis.

Fig. S4 . MALDI-TOF MS spectra of isolate P. veronii PMFKG-R30; the absolute intensities of the ions are shown on the y axis, and the masses (m/z) of the ions are shown on the x-axis.

Fig. S5 . MALDI-TOF MS spectra of isolate P. agglomerans PMFKG-R20; the absolute intensities of the ions are shown on the y axis, and the masses (m/z) of the ions are shown on the x-axis.

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Radojević, I.D., Ćirković, K.G., Grujović, M.Ž. et al. Characterization of Bacterial Isolates from Tailings Pond and Their Resistance to Heavy Metals and Antibiotics. Appl Biochem Microbiol 60, 347–357 (2024). https://doi.org/10.1134/S0003683824020157

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