Subthalamic nucleus but not entopeduncular nucleus deep brain stimulation enhances neurogenesis in the SVZ-olfactory bulb system of Parkinsonian rats

Introduction

Deep brain stimulation (DBS) is a highly effective treatment option in middle- to late-stage Parkinson’s disease (PD), offering long-lasting motor symptom benefits (Deuschl et al., 2013). While motor symptoms improve shortly after DBS onset, new data suggest that there is also a more delayed alleviation of various non-motor symptoms [NMS; (Wolz et al., 2012; Dafsari et al., 2016, 2018)]. The mechanisms behind NMS improvements remain unresolved so far, though an association with location of active electrode contacts has been proposed (Funkiewiez et al., 2003; Petry-Schmelzer et al., 2019). Some NMS, e.g., olfactory dysfunction and depression, can frequently occur as prodromal symptoms (Tolosa et al., 2009) and have been linked to alterations in adult neurogenesis in PD: while for hyposmia, an increase in dopaminergic OB interneurons has been proposed as a pathophysiological mechanisms (Huisman et al., 2004; Mundiñano et al., 2011), hippocampal atrophy in PD has been linked to PD-associated depression and cognitive impairment (Brück et al., 2004; Györfi et al., 2017). Interestingly, according to Braak et al. (2003), the olfactory system is also one of the first regions to be affected by PD neuropathology.

Adult neurogenesis is well-characterized in the neurogenic niches of the subventricular zone (SVZ) of the lateral ventricles and in the dentate gyrus (DG). SVZ-derived adult neural stem/progenitor cells (named herein aNSCs) migrate along the rostral migratory system (RMS) into the olfactory bulb (OB) to differentiate into various interneurons, which play a role in olfactory learning, e.g., odor discrimination (Doetsch et al., 1997; Doetsch, 2003; Breton-Provencher et al., 2009). In contrast, DG-aNSCs contribute to resident neuronal turnover (Jhaveri et al., 2010) and are involved in memory formation and mood regulation (van Praag et al., 1999; Kempermann et al., 2003; Snyder et al., 2005).

In the context of electric stimulation, directed migration of SVZ-aNSCs into the OB is a prerequisite for adequate neuronal turnover and is partly controlled by endogenous electric fields, both in the adult brain and during development (Hotary and Robinson, 1992; Cao et al., 2013, 2015). Experimental direct current electric fields induce cathodal migration without altering aNSC differentiation in vivo, with similar findings after biphasic pulsed stimulation (Iwasa et al., 2019; Sefton et al., 2020). In vitro, various studies demonstrated influences on migration, proliferation and differentiation of aNSCs, though stimulation paradigms, e.g., regarding field strengths and duration of stimulation, vary greatly (Li et al., 2008; Ariza et al., 2010; Babona-Pilipos et al., 2015; Iwasa et al., 2019).

In PD, impairment of adult neurogenesis has been described both in different animal models and in patients: in the 6-OHDA and the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned models, dopaminergic denervation resulted in decreased aNSC proliferation and reduced numbers of mature newborn neurons in the respective target regions in some studies; however, more recent studies challenged these findings with either no or even contrary effects of dopamine depletion on neurogenesis (Höglinger et al., 2004; Aponso et al., 2008; Winner et al., 2009; Ermine et al., 2018). In genetic animal models, e.g., LRRK2 G2019S or mutant and wild-type α-synuclein mice, neurogenesis was overall impaired, though congenital dopamine depletion in Pitx3-mutant mice affected NSC proliferation exclusively in younger animals (Winner et al., 2008, 2011; Marxreiter et al., 2009; Kohl et al., 2016; Brandt et al., 2017). Similar findings were obtained in clinical studies: while Höglinger et al. (2004) described reduced aNSC proliferation in the SVZ in patients’ post-mortem brains, these findings have been challenged in subsequent investigations (van den Berge et al., 2011; Terreros-Roncal et al., 2021).

The impact of chronic DBS on adult neurogenesis has not been investigated in detail so far: Khaindrava et al. (2011) report unaltered SVZ- and DG-aNSC proliferation after eight days of STN-DBS, but increased survival of newborn neuroblasts and neurons in the 6-OHDA rat model. Other studies in healthy animals or in different psychiatric disease models, e.g., for depression and dementia, mostly report an increase in adult-generated hippocampal neurons, independent of the stimulation site (e.g., entorhinal cortex, anteromedial thalamus) or DBS duration (Toda et al., 2008; Stone et al., 2011; Chamaa et al., 2021; Zhou et al., 2022). The only study on aNSC proliferation in PD patients demonstrated an increase in SVZ precursor cell proliferation in DBS-treated patients compared to both unstimulated PD subjects and healthy controls, though without data on hippocampal neurogenesis (Vedam-Mai et al., 2014).

In the present study, we used the 6-OHDA PD rat model to comparatively investigate the influence of long-term continuous DBS in the STN or the entopeduncular nucleus (EPN; rodent homologue of the human internal globus pallidus) on proliferation and differentiation of aNSCs in the SVZ and the DG as a putative mediator of NMS improvement after DBS. To elucidate direct electrical stimulation of aNSCs as a putative mechanism of DBS action on neurogenesis, we not only compare STN-DBS and EPN-DBS but also simulate the volume of tissue activated (VTA) and the corresponding strength of the electric field within the neurogenic regions.

Materials and methods

Animals: All procedures were permitted by responsible authorities (Landesdirektion Sachsen, Germany; reference numbers DD24-5131/207/3) and carried out in line with ARRIVE guidelines and the EU Directive 2010/63/EU for animal experiments as reported previously (Fauser et al., 2021). We used female Wistar rats (∼240–260 g at purchase, Charles River Laboratories, Sulzfeld, Germany) that were kept under a 12 h/12 h light-dark cycle and had ad libitum access to food and water. Rats were housed 2–3/cage until successful lesioning and singularised after electrode implantation to prevent reciprocal electrode and stimulator destruction. Influences of STN-DBS and EPN-DBS on midbrain dopaminergic systems have already been published from the same cohort (Fauser et al., 2021). Induction of the dopaminergic deficit and DBS surgery were carried out as described before (Fauser et al., 2021) and are detailed in the Supplementary Methods Section. We applied bilateral DBS or respective sham stimulation in all animals and used the contralateral, non-lesioned hemispheres as a healthy control.

Thymidine analogs labeling: All animals were injected intraperitoneally (i.p.) with 57.5 mg/kg BW 5′-iodo-2′-deoxyuridine (IdU; 23 mg/ml in 0.9% NaCl with 0.2 N NaOH; MP Biochemicals, CA, USA) every 12 h for a total of 72 h two days after DBS onset; 21 days later, rats received 42.5 mg/kg BW 5′-chloro-2′-deoxyuridine (CldU; 17 mg/ml in 0.9% NaCl; Sigma-Aldrich, Dorset, UK) i.p. with an identical protocol to label proliferating cells in the respective neurogenic regions (Vega and Peterson, 2005).

Immunohistochemistry: For triple immunostaining of every 6th 40 μm section, we used standard protocols as described before [(Hermann et al., 2009), see Supplementary Methods for details] and the following primary antibodies: rat anti-BrdU for detection of CldU incorporation (1:500; RRID:AB_609568; Bio-Rad Laboratories, CA, USA), mouse anti-BrdU for detection of IdU (1:500; RRID:AB_400326; BD Biosciences, Heidelberg, Germany), rabbit anti-TH (1:500; RRID:AB_390204; Chemicon GmbH, Limburg an der Lahn, Germany), chicken anti-NeuN (1:1000; RRID:AB_11155058; Abcam, Berlin, Germany), rabbit anti-GABA (1:1000; RRID:AB_477652; Sigma-Aldrich, Taufkirchen, Germany), goat anti-DCX (1:100; RRID:AB_2088494; Santa Cruz Biotechnology, Heidelberg, Germany), mouse anti-PH3 (1:100; RRID:AB_331748; Cell Signalling Technologies, Danvers, MA, USA). In a previous study, Vega and Peterson demonstrated that one of the BrdU antibodies used in the present experiments (Bio-Rad Laboratories, CA, USA) specifically detects CldU, while the other BrdU antibody (BD Biosciences, Heidelberg, Germany) selectively binds to IdU (Vega and Peterson, 2005).

The following day, sections were incubated in appropriate secondary antibodies; cell nuclei were counterstained with bisbenzimide H33342 fluorochrome trihydrochloride (Invitrogen, CA, USA) or 4′, 6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich, Taufkirchen, Germany).

Imaging and quantifications: Respective OB, SVZ and DG sections were imaged and quantified using a Zeiss Laser scanning confocal LSM 700 or a motorized Axio.Observer.Z1 and ZEN Blue software with Tiles and Position Module (Carl Zeiss, Oberkochen, Germany). We chose standardized settings at the beginning of the experiments, which remained unchanged for all subsequent imaging studies. For all quantifications, the rater was blinded to group allocation of the respective slices. For quantitative histology within the OB, we defined regions of interest (ROIs) and placed counting squares with a set volume of 250 × 250 × 40 μm³ randomly within the glomerular layer (six ROIs/slice) and granule cell layer (two ROIs/slice) of the OB. These counting squares were again placed in a blinded manner by an employee who was not otherwise part of the study. Within the ROIs in the granule cell layer, we quantified CldU⁺GABA⁺ and IdU⁺GABA⁺ cells. Within the ROIs in the glomerular layer, we analyzed CldU⁺TH⁺, IdU⁺TH⁺, CldU⁺GABA⁺, IdU⁺GABA⁺ cells. CldU⁺IdU⁺ cells were extremely rare. Therefore, they were not quantified. Due to comparatively low numbers of labeled proliferating cells, we quantified IdU⁺, CldU⁺, IdU⁺NeuN⁺ and CldU⁺NeuN⁺ cells within the entire DG. For the SVZ-RMS continuum, we defined counting squares (CS) of 300 μm³ × 100 μm³ × 40 μm³ and placed them in the most dorsal parts of the SVZ within the regions of the highest proliferative activity along the dorsal-ventral axis. Within the CSs, we quantified PH3⁺ proliferating cells and performed intensity measurements using ZEN Blue 2.3 software to quantify densely packed DCX⁺ cells. Coronal slices from Bregma −0.48 mm to < 2.52 mm were defined as SVZ, while slices ≥ 2.52 mm were defined as RMS [according to Paxinos and Watson (2007) and Golmohammadi et al. (2008)].

In silico modeling of the electric fields: To adequately characterize the electric field distribution and the volume of tissue activated (VTA) in the rat brain, we used the second version of our open-source simulation platform OSS-DBS (Butenko et al., 2020). For precise modeling of the computational domain, we employed a segmented magnetic resonance image (MRI) from the multidimensional magnetic resonance histology atlas (Johnson et al., 2012). To account for the anisotropic tissue properties of the brain, we use diffusion tensor data estimated in the same space. The dielectric properties of brain tissue, including gray matter, white matter, and cerebrospinal fluid, are estimated based on a the four-dispersion Cole-Cole model (Gabriel et al., 1996). Due to the absence of magnetic induction and the relatively low frequencies, the quasi-static approximation of Maxwell’s equation is valid (van Rienen, 2001). We solved the equation using the finite element method to estimate the potential and the electric field. Thresholding was used to determine the extent of the VTAs. Due to the smaller diameter of axons in rats compared to humans, a relatively high threshold is required to activate them. We chose a threshold of 0.301 V/mm based on our stimulation amplitude and the values proposed by Astrom et al. (2015) for the relationship between electric field distribution and neural activation.

Statistics analyses were carried out with SPSS (version 27, IBM, NY, USA), while data plots and figures were created with GraphPadPrism 9.4.1 (GraphPad Software, CA, USA) and BioRender.com (BioRender, Canada). Shapiro-Wilk test and visual inspection of box plots were applied to test for normal distribution and homogeneity of variances was analyzed with Levene test. Since it cannot be excluded that incorporation of IdU and CldU is variable between the two substances, we did not perform a within-subject analysis for the two injection timepoints, but used t-tests or Mann–Whitney-U-tests for group comparisons for each timepoint separately depending on the normal distribution of our data. All data are presented as boxplots with a central mark at the median, bottom, and top edges of the boxes at 25th and 75th percentiles, respectively, and whiskers at the minimum/maximum (dots represent individual values), except data on cell counts of the SVZ-RMS continuum which are presented as mean ± SEM for clarity.

Results

Chronic dopaminergic deficiency does not persistently alter adult neurogenesis in the 6-OHDA model

First, we analyzed the effects of chronic dopaminergic deficiency in the 6-OHDA model on net neurogenesis as indicated by thymidine analogs labeling in severely lesioned animals with a stable dopaminergic deficit in closely matched groups: the overall decrease in nigral dopaminergic neurons as well as dopaminergic fiber density in the striatum both dropped by ∼90% [published in Figures 1e, f in Fauser et al. (2021)]. In contrast to previous studies, we investigated neurogenesis in animals with a completed dopaminergic deficit and not during the ongoing degeneration shortly after lesioning (Höglinger et al., 2004; Winner et al., 2009). In the present study, there was no persistent effect 15 weeks after induction of the severe dopaminergic deficiency on aNSC proliferation, when comparing the ipsilateral 6-OHDA-lesioned side with the contralateral non-lesioned hemisphere in the OB and DG (see Supplementary Table 1 for detailed statistics). These findings align with clinical studies in PD patients, in which recent studies report unaltered NSC proliferation compared to healthy, age-matched controls (van den Berge et al., 2011; Terreros-Roncal et al., 2021).

Chronic STN-DBS persistently increases SVZ-OB neurogenesis in an animal model of stable dopaminergic deficiency

To assess potential effects of DBS on cellular plasticity within the neurogenic niche of the SVZ-RMS-OB axis, we initially quantified different subtypes of newborn neurons within the glomerular and granule cell layers of the OB from two different time points: early after DBS onset (two days, EARLY) and three weeks later (LATE) with persistent electrical stimulation. Since SVZ-aNSC proliferation seems to be unaffected by DBS (Khaindrava et al., 2011), we here focus on total numbers of terminally differentiated newborn neurons (for details on experimental setups, see Figure 1A).

Figure 1. Effects of deep brain stimulation in the subthalamic nucleus (STN-DBS) on adult neurogenesis in the olfactory bulb (OB) in hemiparkinsonian rats. (A) Schematic representation of experimental paradigm to investigate the effects of STN-DBS vs. DBS in the entopeduncular nucleus (EPN) on adult neurogenesis in the OB and dentate gyrus (DG) in 6-hydroxydopamine (6-OHDA) hemiparkinsonian rats. To secure a stable dopaminergic deficit, bilateral DBS electrodes were implanted eight weeks after initial 6-OHDA lesion either into the EPN or into the STN followed by high frequency stimulation for five weeks with perfusion of animals one week after termination of stimulation. SHAM animals (electrode implantation without active stimulation) served as controls. IdU and CldU were injected six times bi-daily starting two days after DBS onset and three weeks later. Created with BioRender.com. (B,C) Representative immunohistological images of newborn neurons in the OB in STN_SHAM and STN_STIM conditions. IdU (red) labels newborn neurons that were generated early (two days) after DBS initiation, while CldU (green) indicates newborn neurons after three weeks of continuous DBS. GABAergic neurons (pink) are found both in granule cell layers (B) and in glomerular layers (C, lower panel), while TH⁺ dopaminergic neurons (pink) are restricted the latter (C, upper panel). Cell nuclei were counterstained with DAPI. Scale bar, 50 μm. (D) A significant increase in the numbers of newborn GABAergic neurons in the granule cell layer and TH⁺ dopaminergic neurons in the glomerular layer were found both early after STN-DBS onset and three weeks later, while GABAergic neurons in the glomerular layer were only significantly increased immediately after STN-DBS induction compared to sham stimulation. All results were obtained from 6-OHDA-lesioned hemispheres. Data are presented as boxplots with a central mark at the median, bottom, and top edges of the boxes at 25th and 75th percentiles, respectively, and whiskers at the minimum/maximum (dots represent individual values). *represents P < 0.05 and **represents P < 0.01 from Mann–Whitney-U-tests. 6-OHDA, 6-hydroxydopamine; DBS, deep brain stimulation; STN, subthalamic nucleus; EPN, entopeduncular nucleus; OB, olfactory bulb; IdU, 5-iodo-2′-deoxyuridine; CldU, 5-chloro-2′-deoxyuridine; GABA, gamma aminobutyric acid; TH, tyrosine hydroxylase; DAPI, 4′,6-diamidino-2-phenyl-indol-dihydrochloride.

We analyzed numbers of newborn GABAergic neurons in the granule cell layer as well as dopaminergic (TH⁺) and GABAergic neurons in the glomerular layer in the right, 6-OHDA-lesioned hemisphere (Figures 1B–D). We report significant increases in all three cell types early after DBS onset, though these effects persisted only in the granule cell layer and in dopaminergic, but not GABAergic, cells in the glomerular layer after three weeks. Total numbers of newborn GABAergic neurons in the granule cell layer early after DBS onset in the STN_SHAM animals were 10,186 ± 1,106 neurons/mm³ and 14,707 ± 552 neurons/mm³ in STN_STIM animals on the 6-OHDA-lesioned side, while we found 11,869 ± 1,003 and 15,381 ± 987 neurons/mm³ in STN_SHAM and STN_STIM animals after three weeks of continuous STN-DBS (P = 0.006 and P = 0.04).

In the glomerular cell layer, we quantified two different neuronal subtypes, namely dopaminergic (TH⁺) and GABAergic neurons (Figure 1D): in the lesioned hemisphere, we found a significant increase in both subtypes of newborn neurons after STN-DBS compared to sham stimulation after treatment onset, which was, however, only maintained in dopaminergic neurons. Newborn dopaminergic neurons in the glomerular layer early after DBS onset were 224 ± 8 and 330 ± 17 neurons/mm³ in STN_SHAM and STN_STIM animals, respectively, and 218 ± 31 and 358 ± 41 neurons/mm³ under chronic DBS treatment in 6-OHDA-lesioned hemispheres (P = 0.004 and P = 0.04). Numbers of GABAergic neurons were 241 ± 55 and 439 ± 51 neurons/mm³ early after DBS onset in STN_SHAM and STN_STIM cohorts and 286 ± 64 and 294 ± 30 neurons/mm³ during long-term DBS in lesioned hemispheres (P = 0.04 and P = 0.91; Figure 1D).

In addition, we quantified newborn neurons in contralateral non-lesioned hemispheres. Here, only numbers of newborn dopaminergic neurons in the glomerular cell layer were significantly increased with 286 ± 27 and 524 ± 80 neurons/mm³ in STN_SHAM and STN_STIM animals early after DBS onset and 307 ± 50 and 608 ± 95 neurons/mm³ after chronic treatment (P = 0.03 and P = 0.04, respectively; for complete data see Supplementary Figures 1A–C and Supplementary Table 2).

An increase in OB dopaminergic neurons in bilateral EPN-DBS is not maintained during chronic stimulation

Prompted by previous studies on STN- and EPN-DBS (or Gpi-DBS in humans), in which dopaminergic plasticity was restricted to STN-DBS (Fischer et al., 2015; Fauser et al., 2021), we were interested whether the different stimulation targets also have differential effects on adult neurogenesis. Again, we quantified newborn GABAergic and dopaminergic neurons in the granule and glomerular layers of the OB. We only found a significant increase in numbers of newborn dopaminergic neurons in 6-OHDA-lesioned hemispheres in the OB early after DBS onset, which was, however, not maintained during chronic DBS (P = 0.003; Figure 2, Supplementary Figure 1D and Supplementary Table 2).

Figure 2. Effects of deep brain stimulation in the entopeduncular nucleus (EPN-DBS) on adult neurogenesis in the olfactory bulb (OB) of hemiparkinsonian rats. (A,B) Representative immunohistological images of newborn neurons the OB in EPN_SHAM and EPN_STIM conditions. IdU (red) labels newborn neurons that were generated early (two days) after DBS onset, while CldU (green) indicates newborn neurons after three weeks of continuous DBS. GABAergic neurons (pink) were found both in the granule cell layer (A) and in the glomerular layer (B, lower panel), while TH⁺ dopaminergic neurons (pink) were restricted to the latter (B, upper panel). Cell nuclei were counterstained with DAPI. Scale bar, 50 μm. (C) In 6-OHDA-lesioned hemispheres, bilateral EPN-DBS did not alter the numbers of newborn GABAergic neurons in the granule cell layer or glomerular layer compared to sham stimulation, but increased dopaminergic neuron counts in the glomerular layer early after DBS onset; however, this was not maintained after three weeks. Data are presented as boxplots with a central mark at the median, bottom, and top edges of the boxes at 25th and 75th percentiles, respectively, and whiskers the minimum/maximum (dots represent individual values). **represents P < 0.01 from Mann–Whitney-U-tests. DBS, deep brain stimulation; EPN, entopeduncular nucleus; OB, olfactory bulb; IdU, 5-iodo-2′-deoxyuridine; CldU–5-chloro-2′-deoxyuridine; GABA, gamma aminobutyric acid; TH, tyrosine hydroxylase; DAPI–4′,6-diamidino-2-phenyl-indol-dihydrochloride.

Chronic STN- and EPN-DBS does not influence hippocampal neurogenesis

Impairment of hippocampal neurogenesis has been reported to be associated with the development of mood disorders and cognitive decline (Winocur et al., 2006; Zhou et al., 2022), two highly prevalent NMS in PD (Rodriguez-Blazquez et al., 2020). In our study, bilateral STN-DBS did not provoke any alterations in the numbers of newborn neurons generated shortly after DBS onset in the subgranular zone (SGZ; P = 0.16) or granular zone (GZ; P = 0.50) and three weeks later in 6-OHDA lesioned hemispheres (P = 0.53 and P = 0.58). In addition, STN-DBS did not alter neurogenesis in stimulated, non-lesioned hemispheres compared to sham stimulation. Similar findings were obtained in EPN-DBS (see Supplementary Figure 2 and Supplementary Tables 1, 2 for details).

STN-DBS does not persistently increase SVZ-aNSC proliferation beyond the stimulation period

Since DBS had to be terminated one week prior to perfusion due to technical limitations, we next assessed SVZ-aNSC proliferation in 6-OHDA-lesioned animals without active DBS. In a previous study, STN-DBS effects persisted beyond the stimulation period by several weeks (Fauser et al., 2021). We quantified proliferating PH3⁺ aNSCs and DCX⁺ neuroblasts in the SVZ and the entire RMS in STN_SHAM and STN_STIM animals. Animals from EPN groups were not further analyzed. Dense DCX⁺ neuroblasts were quantified using intensity measurements (Supplementary Figures 3A, B). We found no significant differences in the total number of PH3⁺ proliferating aNSCs in non-lesioned and lesioned hemispheres within the entire SVZ (P = 0.56 and P = 0.11) and RMS (P = 0.41 and P = 0.91), nor in the density of DCX⁺ neuroblasts between STN_SHAM and STN_STIM animals in the entire SVZ (both P = 0.56) and RMS (P = 0.12 and P = 0.89; all from Mann–Whitney-U-tests; Supplementary Figures 3C, D), respectively.

The VTA of either DBS target does not encompass the neurogenic regions in the SVZ or DG, respectively

Since the process of neurogenesis is in part regulated by endogenous electric fields both in vitro and in vivo (Iwasa et al., 2019; Sefton et al., 2020), we analyzed the actual electric field strengths within the neurogenic region of the SVZ. Volumes of tissue activated (VTAs) were calculated with OSS-DBS, showing the rapid decrease of the electric field strengths around the electrode tip (Figure 3, Supplementary Figure 4 and Supplementary Videos 1, 2). Using a threshold of 0.301 V/mm, as explained in the “Materials and methods” section, the VTAs had variable radii depending on the direction with brain morphology but were clearly below 1.5 mm.

Figure 3. Distribution of the electric fields induced by STN-DBS and EPN-DBS. (A,B) Electrode reconstruction within the (A) STN (yellow structure) and (B) EPN (blue structure) in transversal views illustrating the volume of tissue activated (VTA, circumferential red circle around the electrodes) with respect to the two neurogenic niches, namely the SVZ adjusted to the lateral ventricles of the ventricular system (dark green structure) and the dentate gyrus (bright green structure). To illustrate the distance of the VTA to the neurogenic niches in 3D, please refer to Supplementary Videos 1, 2. (C,D) Sectional view of the electric field strength in V/mm during (C) STN-DBS and (D) EPN-DBS. The electrode position relative to STN (yellow structure) and EPN (blue structure) are shown in transversal sections. The color code represents the electric field magnitude in V/mm. The given distance scale (mm) shows that the electric field strength decreases rapidly. (E,F) The electric field strength is estimated over the shortest distance between the electrode tip and the neurogenic region. It is shown in blue for (E) STN-DBS and (F) EPN-DBS with an error margin (light blue area) representing the electric field strength for an electrode placed ± 1 mm in the direction of the SVZ. The green area spans the neurogenic region of the SVZ that was analyzed here (from bregma –0.48 to 2.52 mm). The horizontal red dashed line indicates the chosen activation threshold based on the work of Astrom et al. (2015). DBS, deep brain stimulation; EPN, entopeduncular nucleus; STN, subthalamic nucleus; VTA, volume of tissue activated.

Further, the magnitude of the electric field has been estimated on the shortest path between the stimulation target (electrode tip) and the neurogenic region, which may not show the highest field strengths but clearly showed that the impact of the electric field in the target region is negligibly small (Figures 3E, F). Comparing STN-DBS against EPN-DBS, no differences in the electric field magnitude in the neurogenic regions could be observed.

Discussion

The present study indicates that bilateral long-term STN-DBS increases forebrain neurogenesis within the SVZ-RMS-OB continuum in a 6-OHDA hemiparkinsonian rodent model of stable dopaminergic deficiency. These neuroplasticity effects are specific for both the stimulation site, i.e., not reproducible with EPN-DBS, and the respective neurogenic niche, as hippocampal neurogenesis was entirely unaltered. In healthy, non-lesioned hemispheres, STN-DBS also enhanced the numbers of newly-generated dopaminergic interneurons in the OB. In addition, we are the first to show that these STN-DBS-mediated alterations in neurogenesis persist during long-term DBS. Since we compared active with sham DBS, subthalamotomy-like effects of electrode placement into the STN do not explain the STN-DBS actions on neurogenesis.

In contrast to previous studies (Höglinger et al., 2004; Winner et al., 2009; Khaindrava et al., 2011), our model of severe, but—at DBS onset—already completed dopaminergic deficiency did not show alterations in aNSC proliferation in the neurogenic niches when compared to non-lesioned hemispheres. The main differences between these studies and ours are the site of lesioning and the interval between neurotoxic lesioning and labeling of proliferating aNSCs. Regarding the time interval, a direct toxic effect of the applied substances on aNSC proliferation in previous studies could be discussed as well as neuroimmunological mechanisms involving microgliosis and reactive astrogliosis, which also resolve over time (Marinova-Mutafchieva et al., 2009; Walsh et al., 2011; Fauser et al., 2021). However, our findings are in line with our previous study in a genetic Pitx3-mutant PD model demonstrating that embryonic degeneration of midbrain dopaminergic neurons affects early postnatal but not late neurogenesis in both the SVZ and hippocampus (Brandt et al., 2017). Furthermore, some human studies describe unaltered aNSC proliferation in PD patients with a long disease duration compared to age-matched controls (van den Berge et al., 2011; Terreros-Roncal et al., 2021). Nevertheless, increased forebrain neuronal turnover might still lead to beneficial effects concerning related NMS, e.g., hyposmia, as it has already been described in a clinical study (Saatçi et al., 2019).

Our study is the first to describe that EPN-DBS does not alter cellular plasticity in any of the neurogenic regions. In line with these findings, we and others already reported a lack of plasticity in the dopaminergic systems after EPN-DBS, which had been repeatedly demonstrated after STN-DBS (Khaindrava et al., 2011; Fischer et al., 2015; Fauser et al., 2021). We hypothesize that mechanisms of action of DBS might differ between stimulation sites, since e.g., DBS in different limbic targets for psychiatric conditions consistently increases hippocampal plasticity, which has not been modulated at all in our study (Toda et al., 2008; Stone et al., 2011; Chamaa et al., 2021). Regarding comparative studies on EPN-(or GPI-DBS in humans) and STN-DBS in PD, the pattern of associated motor and non-motor improvements seems to differ between the stimulation targets (Odekerken et al., 2013; Dafsari et al., 2020), while overall cognitive and psychiatric outcomes are equivalent over time (Boel et al., 2016).

Growing evidence from both in vitro and in vivo studies demonstrates that aNSCs are electrosensitive cells (Babona-Pilipos et al., 2011; Iwasa et al., 2017; Sefton et al., 2020). However, our in silico studies on the electric field strength using DBS parameters used in clinical settings for PD demonstrated that the VTA of both stimulation targets does not include the neurogenic niches. Indeed, the electric field strength acting on the aNSC niches in our studies using clinical DBS parameters is much smaller (< 0.01 V/mm) than those used to study aNSC properties in vitro [e.g., 250 mV/mm during the cathodal pulse of asymmetric biphasic stimulation applied to the striatum for only 1 h; (Iwasa et al., 2019; Sefton et al., 2020)]. Of note, a detailed simulation of the electric field strength actually acting on the aNSCs within their niches was not reported in previous studies. However, a direct comparison between our stimulation parameters between these studies is not feasible, due to widely different technical paradigms, e.g., electrodes, stimulation targets and parameters, and the duration of stimulation.

Moreover, according to our simulations of the electric fields and corresponding VTAs, the specific effects of STN-DBS compared to EPN-DBS are not sufficiently explained by differences in local electric field strengths within the respective neurogenic niches. Together, direct electrical stimulation of the neurogenic niche as a mechanism of STN-DBS effects on SVZ-OB neurogenesis is highly unlikely. In contrast, the specificity of the neurogenic effects of STN-DBS with respect to the stimulation target is a strong argument for an indirect stimulation of direct or indirect afferents to the SVZ neurogenic niche as already shown for hippocampal neurogenesis enhanced by electrical stimulation of hippocampal afferents (Derrick et al., 2000; Bruel-Jungerman et al., 2006; Toda et al., 2008; Encinas et al., 2011; Stone et al., 2011). However, the corresponding neurogenic network regulating SVZ-OB neurogenesis (Chen et al., 2023) with regard to STN-DBS effects remains to be determined, especially since direct interconnections between the STN and the medial part of the striatum/SVZ are only sparse (Groenewegen and Berendse, 1990; Schmitt et al., 2016).

In a single previous study on DBS actions on adult neurogenesis in the 6-OHDA model, the authors reported an increased survival of newborn neuroblasts after eight days of STN-DBS within the OB and the DG without concordant alterations in aNSC proliferation rates (Khaindrava et al., 2011). The authors concluded that these neurogenic effects of short-term STN-DBS are not directly linked to dopamine function. In our study, long-term STN-DBS did also not persistently alter aNSC proliferation in the SVZ/RMS. Whether STN-DBS instead acts either on aNSC differentiation or neuroblast survival needs to be elucidated in future studies.

Our study is limited regarding its small animal numbers due to the very high experimental effort in such cohorts; however, the final group sizes are in the range of similar experimental DBS studies (Spieles-Engemann et al., 2011; Fischer et al., 2017; Musacchio et al., 2017). We tried to minimize this limitation by using closely matched groups according to their amphetamine-induced rotational behavior, which sufficiently correlates with between substantia nigra pars compacta (SNpc) dopaminergic cell loss (Tronci et al., 2012). Moreover, we used the contralateral, non-lesioned hemisphere as a control condition and did not include additional healthy or sham-lesioned animals. Therefore, we cannot exclude that neurogenesis might already be altered in these animals due to the contralateral lesion; however, previous studies did not detect differences in contralateral neurogenesis compared to sham-lesioned animals (Höglinger et al., 2004; Suzuki et al., 2010; Fricke et al., 2016). In addition, we included only female animals, limiting our results’ generalizability. Previous studies demonstrated alterations in 6-OHDA susceptibility, as well as influences of sex hormones on adult neurogenesis in female mice compared to their male counterparts and (Tanapat et al., 1999; Somensi et al., 2021). However, our study therefore contributes to a limited number of studies in female animals (Beery and Zucker, 2011), in which influences of sex and sex hormones on adult neurogenesis remain contradictory (Tanapat et al., 1999; Lagace et al., 2007; Yagi and Galea, 2019). In translational terms, the 6-OHDA model does not adequately reflect the neuropathological and clinical hallmarks of human PD, e.g., α-synuclein accumulation and a chronic progressive disease course (Braak et al., 2003). This might limit the transferability of our results into clinical application. However, even most rodent models of synucleinopathies do not present with a similar degree of midbrain dopaminergic degeneration as PD patients (Kordower et al., 2013; Nuber et al., 2013; Polissidis et al., 2021).

It has been previously reported that increased numbers of dopaminergic interneurons in the OB might actually be associated with impaired olfactory function in different neurodegenerative diseases (Huisman et al., 2004; Mundiñano et al., 2011); therefore, our findings of an enhancement in TH⁺ OB neuron counts (in both dopamine-depleted and healthy hemispheres) might even have detrimental effects. Behavioral studies are therefore required to verify the assumption that modulations of neurogenesis might influence certain NMS, but also to better understand DBS influences in healthy subjects, as our results indicate concordant effects, e.g., on the generation of dopaminergic interneurons in the OB, in both healthy and dopamine-depleted conditions. However, such behavioral studies cannot be conducted in a unilateral model of dopaminergic deficiency, as the unaffected hemisphere partly fills in for the unilateral deficit. This has already been shown in behavioral studies on olfaction in 6-OHDA rats, in which no signs of hyposmia could be detected (Ilkiw et al., 2019; Alberts et al., 2022). However, other NMSs, such as anhedonia or depression, are present in unilaterally lesioned animals (Ilkiw et al., 2019). Therefore, we recommend further studies in animals with generalized neuropathology, e.g., transgenic animals with α-synuclein overexpression, but also in healthy control subjects to further dissect the specificity of DBS effects in neurodegenerative diseases.

Conclusion

Our study provides first evidence that long-term STN-DBS persistently increases SVZ-OB neurogenesis, but not hippocampal neurogenesis in the 6-OHDA PD model of severe, unilateral dopaminergic deficiency. These effects are stimulation-site specific and cannot be reproduced with EPN-DBS, which is, however, equally efficient in treating motor symptoms in PD patients. Further studies to determine behavioral benefits of increased neurogenesis on forebrain-related NMS, e.g., olfaction and hedonistic behavior, need to be carried out, possibly in animal models that more closely resemble the human disease, e.g., models of synucleinopathy. These findings might help to optimize DBS therapy concerning NMS, which often reduce the quality of life to a greater extent than PD motor impairments.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement

The animal study was approved by the Landesdirektion Sachsen, Germany; reference number DD24-5131/207/3. The study was conducted in accordance with the local legislation and institutional requirements.

Author contributions

MF: Funding acquisition, Investigation, Methodology, Visualization, Writing – original draft. JP: Methodology, Software, Visualization, Writing – original draft. HW: Investigation, Methodology, Writing – review & editing. MS: Formal analysis, Investigation, Writing – review & editing. CW: Conceptualization, Resources, Writing – review & editing. RH: Methodology, Supervision, Writing – review & editing. RA: Funding acquisition, Methodology, Software, Supervision, Writing – review & editing. UvR: Conceptualization, Funding acquisition, Supervision, Writing – review & editing. MB: Conceptualization, Methodology, Supervision, Writing – review & editing. AS: Conceptualization, Formal analysis, Supervision, Visualization, Writing – review & editing.

Funding

The authors declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Collaborative Research Centre CRC 1270 “Electrically Active Implants” (DFG; SFB 1270/1 and 1270/2—299150580) to MF, RA, and UvR. MF was supported by the Else Hirschberg Women’s Advancement Program of the University Medical Center Rostock. The funders neither played a role in the study design, conduct, data collection, analysis or interpretation, nor participated in the manuscript’s preparation, review or approval.

Acknowledgments

We gratefully acknowledge the fruitful scientific discussions with Dr. Franz Markert, University of Rostock. We thank Vivien Charlotte Retzlaff for their help in DBS electrode localisation. We thank Sylvia Kanzler, Andrea Kempe, and Uta Naumann for their technical support and help with immunohistochemical analyses.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The authors declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

Publisher’s note

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Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fncel.2024.1396780/full#supplementary-material

References