Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library

Iou Ven Chang; Hiroshi Tsutsumi; Hisakazu Mihara

doi:10.1039/C7MB00495H

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Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library†

Iou Ven Chang,^a Hiroshi Tsutsumi

^b and Hisakazu Mihara

*^b

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* Corresponding authors

^a Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259-B40, Midori-ku, Yokohama 226-8501, Japan

^b School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta-cho 4259-B40, Midori-ku, Yokohama 226-8501, Japan
E-mail: hmihara@bio.titech.ac.jp

Abstract

Mannose-modified lectin-binding peptides were obtained from an α-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies described in this study will enhance the selection of saccharide-modified ligands through the synergistic effects of sugar and peptide units, with better specificity and affinity towards lectin proteins.

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Article information

DOI: https://doi.org/10.1039/C7MB00495H
Article type: Communication
Submitted: 09 Aug 2017
Accepted: 13 Sep 2017
First published: 13 Sep 2017

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Mol. BioSyst., 2017,13, 2222-2225

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Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library

I. Ven Chang, H. Tsutsumi and H. Mihara, Mol. BioSyst., 2017, 13, 2222 DOI: 10.1039/C7MB00495H

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Molecular BioSystems

Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library†

Abstract

Supplementary files

Article information

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Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library

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