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个人简介

温孚江,男,1955年9月生,山东龙口人。博士,教授,博士生导师。现任山东农业大学校长,全国人大常委,山东省人大副主任。主要从事植物分子病毒学及植物抗病虫基因工程研究。“九五”以来先后主持了国家自然科学基金、美国洛克菲勒基金会资助的项目、国家转基因植物研究及产业化专项、农业部“九五”重点公关项目以及省部级科研课题20项;获省科技进步二等奖2项;在国内外学术刊物上发表研究论文100余篇;主编和副主编国家“十二五”规划教材3部;培养博士、硕士研究生近50人。先后被选拔为“山东省专业技术拔尖人才,授予“中青年有突出贡献专家”、“全国优秀工作者”、“山东省优秀思想政治教育工作者”、“山东省优秀青年知识分子”、“山东省高校十大优秀教师”、“1995年全国优秀教师”等荣誉称号,获国务院“政府特殊津贴”,当选为国家“百千万人才工程”第一二层人才。 教学工作 承担本科生《生物技术研究进展》;博士研究生《分子植物病理学》等课程的教学工作

研究领域

研究方向 1.植物病毒分子生物学 2.植物抗病虫基因工程

科研项目 1.美国洛克非勒基金《水稻基因转化方法及抗虫基因工程研究》,1993-1999. 2.国家自然科学基金《外源基因在转基因植物内遗传规律的研究》(No.39470402) 3.国家自然科学基金《RNA-介导的高抗和多抗植物病毒病育种新策略的研究》(No.39970485) 4.国家转基因植物研究与产业化专项《利用Bt和Xa21基因选育黄淮稻区优质高产双抗(白叶枯、螟虫)水稻新品种国家转基因》(No.J99-B-018) 5.国家自然科学基金《RNA介导的病毒抗性的产生、信号传导及应用研究》(No.30270875) 6.国家转基因植物研究与产业化专项《优质、抗病虫转基因水稻新品种选育》(JY-03-B-13) 7.国家自然科学基金《hpRNA的茎环长度和比例对RNA介导病毒抗性的影响》(No.30471139) 8.国家高技术研究发展计划(863计划)《利用RNA干扰技术培育抗水稻条纹病毒的水稻新种质》(No.2007AA10Z417) 9.转基因生物新品种培育重大专项《抗病转基因水稻新品种培育》(No.2008ZX08001-002,No.2011ZX08001-002;No.2013ZX08001-002;No.2014ZX08001-002)

近期论文

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1.X.Xie,Y.Song,X.Liu,S.Wang,C.Zhu*,F.Wen*.Differenttargetgenesandchimeric-genehairpinstructuresaffectvirusresistancemediatedbyRNAsilencingintransgenictobacco.BiologiaPlantarum,2014,58(3):575-581. 2.YunzhiSong,QuanjunHan,FangJiang,RunzeSun,ZhihangFan,ChangxiangZhu*,FujiangWen*.EffectsofthesequencecharacteristicsofmiRNAsonmulti-viralresistancemediatedbysingleamiRNAsintransgenictobacco.PlantPhysiologyandBiochemistry,2014,77:90-98. 3.JiangY,SunL,JiangM,LiK,SongYandZhuC.Productionofmarker-freeandRSV-resistanttransgenicriceusingatwinT-DNAsystemandRNAi.J.Biosci,2013,38:1–9. 4.RunzeSun,ZhaonanSun,XueyingXie,ChangxiangZhu,FujiangWen*.DifferentAbilitiesofDeliveryWaysfordsRNAExpressedbyBacteriatoProtectTobaccofromCo-infectionwithPVYandTMV.JournalofAgriculturalScienceandApplications.2013,2(2):57-60. 5.L.Zhang,X.Xie,Y.Song,F.Jiang,C.Zhu,F.Wen.ViralresistancemediatedbyshRNAdependsonthesequencesimilarityandmismatchedsitesbetweenthetargetsequenceandsiRNA.BiologiaPlantarum,2013,57(3):547-554. 6.C.Zhang,Y.Song,F.Jiang,G.Li,Y.Jiang,C.Zhu*,F.Wen*.VirusresistanceobtainedintransgenictobaccoandricebyRNAinterferenceusingpromoterswithdistinctactivity.BiologiaPlantarum,2012,56(4):742-748. 7.TongtongYuan,YunzhiSong,KaidongLi,QuanjunHan,ChangxiangZhu*,FujiangWen*.Characterizationof10tobaccoveinbandingmosaicvirusisolatesfromChina.Actavirologica,2012,56:19–24. 8.FangJiang,YunzhiSong,QuanjunHan,ChangxiangZhu*,FujiangWen*.,ThechoiceoftargetsiteiscrucialinartificialmiRNA-mediatedvirusresistanceintransgenicNicotianatabacum.PhysiologicalandMolecularPlantPathology,2011,76:2-8. 9.F.Jiang,B.Wu,C.Zhang,Y.Song,H.An,C.Zhu*,F.Wen*.SpecialoriginofstemsequenceinfluencetheresistanceofhairpinexpressingplantsagainstPVY.BiologiaPlantarum,2011,55(3):528-535. 10.JinMa,YunzhiSong,BinWu,MingsongJiang,KaidongLi,ChangxiangZhu*,FujiangWen*.ProductionoftransgenicricenewgermplasmwithstrongresistanceagainsttwoisolationsofRicestripevirusbyRNAinterference.TransgenicRes,2011,20:1367–13772.754 11.ZhaonanSun,GuohuaYin,YunzhiSong,HailongAn,ChangxiangZhu*,FujiangWen*.BacteriallyExpressedDouble-StrandedRNAsagainstHot-SpotSequencesofTobaccoMosaicVirusorPotatoVirusYGenomeHaveDifferentAbilitytoProtectTobaccofromViralInfection.ApplBiochemBiotechnolBiotechnol,2010,162:1901-1914. 12.XuemeiChen,JingLiu,LiXu,FangJiang,XueyingXie,ChangxiangZhu*,FujiangWen*.InhibitingVirusInfectionbyRNAInterferenceoftheEightFunctionalGenesofthePotatoVirusYGenome.JPhytopathol,2010,158:776–784. 13.ZhaonanSun,YunzhiSong,GuohuaYin,ChangxiangZhuandFujiangWen.HpRNAsDerivedfromDifferentRegionsoftheNIbGeneHaveDifferentAbilitiestoProtectTobaccofromInfectionwithPotatoVirusY.JPhytopathol,2010,158:566–568. 14.LiXu,YunZhiSong,JunHuaZhu,XingQiGuo,ChangXiangZhu*,FuJiangWen*.ConservedSequencesofReplicaseGene-MediatedResistancetoPotyvirusthroughRNASilencing.J.PlantBiol.2009,52:550–559. 15.GuohuaYin,ZhaonanSun,NanLiu,LinZhang,YunzhiSong,ChangxiangZhu*,FujiangWen*.Productionofdouble-strandedRNAforinterferencewithTMVinfectionutilizingabacterialprokaryoticexpressionsystem.ApplMicrobiolBiotechnol,2009,84:323–333. 16.ChangxiangZhu,YunzhiSong,GuohuaYin,FujiangWen*.InductionofRNA-MediatedMultipleVirusResistancetoPotatoY,TomatomosaicandCucumbermosaicviruses.JournalofPhytopathology,2009,157,101–107. 17.ZhangTingting,SongYunzhi,LiuYudong,GuoXingqi,ZhuChangxiang*,WenFujiang*.OverexpressionofphospholipaseDαgeneenhancesdroughtandsalttoleranceofPopulustomentosaChineseScienceBulletin,2008,53(23):3656-3665. 18.H.M.Liu1,C.X.Zhu,X.P.Zhu,X.Q.Guo,Y.Z.SongandF.J.Wen*.ALinkbetweenPVYNCPGene-mediatedVirusResistanceandTransgeneArrangement.J.Phytopathology,2007,155,676–682. 19.YipeiWei,FangyinYao,ChangxiangZhu,MingsongJiang,GuangxianLi,YunzhiSong,FujiangWen*.Breedingoftransgenicricerestorerlineformultipleresistanceagainstbacterialblight,stripedstemborerandherbicide.Euphytica,2008,163:177-184. 20.ZhuJunhua,ZhuXiaoping,WenFujing,BaiQingrong,ZhuChangxiang,SongYunzhi.EffectofcDNAfragmentsindifferentlengthderivedfrompotatovirusYcoatproteingeneontheinductionofRNA-mediatedvirusresistance.ScienceinChinaSer.CLifeSciences,2004,47(4):382-388. 21.ZhuChangxiang,YaoFangyin,LiGuangxian,WenFujiang*.IdentificationofBt-transgenicRicePlantsforResistancetoStripeStemBorer(Chilosuppressalis)andGeneticAnalysisoftheTransgenes.AgriculturalSciencesInChina,2002,1(4):388-390. 22.BoogaartTomvande,WenFJ,DaviesJW,LomonossoffGP.Replicase-derivedresistanceagainstpeaearlybrowningvirusinNicotianabenthamianaisanunstableresistancebaseduponposttranscriptionalgenesilencing.MPMI.2001,14(2):196-203. 23.Huq,E.,Hrrington,S.,Hlossain,M.A.,Wen,F.,McCouch,S.R.,andHodges,T.K.Molecularcharacterizationofpdc2andmappingofthreepdcgenesfromrice.TheorApplGenet,1999,98:815-824. 24.Peng,J.,Wen,F.,Lister,R.M.andHodges,K.K.InheritanceofgusAandneogenesintransgenicrice.PlantMolecularBiology,1995,27:91-104. 25.Peng,J.,Wen,F.,andHodges,K.K.ArapidmethodforqualitativeassayofbothNPTIIandGUSactivitiesintransgenicplants.PlantMolecularBiologyReporter,1993,11:38-47. 26.Bhattacharyya-Pakrasi,M.,Peng,J.,Elmer,J.S.,Laco,G.,Shen,P.,Kaniewska,M.B.,Kononowicz,H.,Wen,F.,Hodges,T.K.,andBeachy,R.N.Specificityofapromoterfromthericetungrobacilliformvirusforexpressioninphloemtissues.ThePlantJournal,1993,4:71-79. 27.Wen,F.,Peng,J.,Lister,R.M.,andHodges,T.K.Aprocedureforregenerationofindicaandjaponicavarietiesofricefromprotoplasts.PlantMolecularBiologyReporter,1992,9:308-321. 28.Azimi,N.T.,Wen,F.,Lister,R.M.,Chen,D.A.andLytle,F.E.Enzyme-linkedimmunoassaysusingnanosecondfluorometricdetection.AppliedSpectroscopy,1992,46:994-998. 29.Wen,F.,Lister,R.M.,andFattouh,F.B.Crossprotectionamongstrainsofbarleyyellowdwarfvirus.J.Gen.Virol.,1991,72:791-799. 30.Wen,F.andLister,R.M.Heterologousencapsidationamongfourisolatesofbarleyyellowdwarfvirus.J.Gen.Virol,1991,72:2217-2223. 31.Wen,F,andLister,R.M.Genomicmaskingandphenotypicmixingbetweenbarleyyellowdwarfviruses.BarleyYellowDwarfNewsletter,1991,4:38-39. 32.Vincent,J.R.,Lister,R.M.,Ueng,P.P.,Wen,F.,Lei,C.H.,Klein,R.E.,andLarkins,B.A.Genomicorganizationofbarleyyellowdwarfviruses.BarleyYellowDwarfNewsletter,1990,3:34-37. 33.Wen,F,andLister,R.M.StudiesofcrossprotectionamongstrainsofBYDV.BarleyYellowDwarfNewsletter,1990,3:37-38. 34.Wen,F,andLister,R.M.Crossprotectionfrombarleyyellowdwarfviruses.BarleyYellowDwarfNewsletter,1989,2:62-64. 35.Wen,F.andLister,R.M.Heterologousencapsulationinmixedinfectionsamongthreeisolatesofbarleyyellowdwarfvirus.Phytopathology,1989,80:1036-1042. 36.Wen,F.andLister,R.M.Crossprotectionandrelationshipsamongbarleyyellowdwarfviruses.Phytopathology,1989,79:1174-1180.

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