Triplophysa hsutschouensis is an endemic species in China, that is mainly distributed in the Hexi Corridor of Gansu Province. Studies on molecular markers in this species are limited. 45 single nucleotide polymorphism markers were developed and characterized in T. hsutschouensis for the first time using restriction site-associated DNA tag sequencing. The minor allele frequency ranged from 0.033 to 0.500, and the observed and expected heterozygosities ranged from 0.067 to 0.600 and 0.066 to 0.508, respectively. The polymorphism information content ranged from 0.062 to 0.375. Among these SNPs, two loci showed significant departures from Hardy–Weinberg equilibrium after Bonferroni correction. The novel polymorphic SNPs will be helpful for further population genetic analysis and natural resource conservation.

三叶草是我国特有种，主要分布于甘肃省河西走廊。对该物种的分子标记的研究是有限的。45个单核苷酸多态性标记在T. hsutschouensis中被开发和表征首次使用限制性位点相关的 DNA 标签测序。次要等位基因频率范围为 0.033 至 0.500，观察和预期杂合度分别为 0.067 至 0.600 和 0.066 至 0.508。多态性信息含量范围为0.062～0.375。在这些 SNP 中，两个位点在 Bonferroni 校正后显示出与 Hardy-Weinberg 平衡的显着偏离。新的多态性SNP将有助于进一步的群体遗传分析和自然资源保护。