In this study, a water-soluble polysaccharide from Eucommia folium was extracted by hot water and purified using Sephadex G-200 gel columns. The results showed that the purified fraction (EFP) has a molecular weight of 9.98 × 10⁵ Da and consisted of rhamnose, arabinose, galactose, glucose, mannose, xylose, galacturonic acid, and glucuronic acid (molar ratio: 0.226: 1.739: 2.183: 1: 0.155: 0.321: 0.358: 0.047). The combination of infrared spectroscopy and NMR analysis proved that EFP is an acidic polysaccharide whose main chain consists of α-L-Araf-(1 → , → 3,5)-α-Araf-(1 → , → 3)-β-Galp-(1 → , → 3,6)-β-Glcp-(1 → , → 2)-α-D-Manp-(1 → , → 4)-α-GalpA-(1 → , → 2,4)-α-Rhap-(1 → . In addition, the in vivo antitumoral activity of EFP was studied using a H22 tumor-bearing mice model. EFP effectively inhibited tumor growth in mice following intragastric administration. By Combining with the results of the apoptosis assay and JC-1 staining analysis, we confirmed that EFP induces apoptosis through the mitochondrial pathway. Furthermore, cell cycle analysis demonstrated that EFP blocks the cell cycle at S phase.

Graphical Abstract

中文翻译：

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一种来自杜仲叶的水溶性多糖：结构表征和体内抗肿瘤活性

在本研究中，通过热水提取杜仲叶中的一种水溶性多糖，并使用 Sephadex G-200 凝胶柱进行纯化。结果表明，纯化级分（EFP）的分子量为9.98×10 ⁵  Da，由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖、半乳糖醛酸和葡萄糖醛酸组成（摩尔比：0.226：1.739：2.183） ：1：0.155：0.321：0.358：0.047）。结合红外光谱和核磁共振分析证明EFP是一种酸性多糖，其主链由α-L-Ara f -(1 → , → 3,5)-α-Ara f -(1 → , → 3)-组成β-Gal p -(1 → , → 3,6)-β-Glc p -(1 → , → 2)-α-D-Man p -(1 → , → 4)-α-Galp A-(1 → , → 2,4)-α-Rha p -(1 → . 此外，使用 H22 荷瘤小鼠模型研究了 EFP 的体内抗肿瘤活性。EFP 有效抑制了小鼠的肿瘤生长结合细胞凋亡试验和JC-1染色分析的结果，我们证实EFP通过线粒体途径诱导细胞凋亡。此外，细胞周期分析表明EFP阻断S期细胞周期。

图形概要