A low-oxygen (hypoxia) tumor microenvironment can facilitate chemotherapy and radiation therapy resistance in tumors and is associated with a poor prognosis. Hypoxia also affects PCa (prostate cancer) phenotype transformation and causes therapeutic resistance. Although O-glycans are known to be involved in the malignancy of various cancers under hypoxia, the expression and function of O-glycans in PCa are not well understood. In this study, the saccharide primer method was employed to analyze O-glycan expression in PCa cells. Results showed that the expression of sTn antigens was increased in PCa cells under hypoxia. Furthermore, it was found that ST6GalNAc1, the sTn antigen synthase gene, was involved in the migration–proliferation dichotomy and drug resistance in PCa cells under hypoxia. The results of this study will contribute to the development of novel diagnostic markers and drug targets for PCa under hypoxia.

低氧（缺氧）肿瘤微环境可促进肿瘤对化学疗法和放射疗法的耐药性，并与不良预后相关。缺氧还会影响 PCa（前列腺癌）表型转化并导致治疗耐药性。尽管已知O-聚糖参与了缺氧条件下多种癌症的恶性发展，但O-聚糖在 PCa 中的表达和功能尚不清楚。本研究采用糖引物法分析PCa细胞中O-聚糖的表达。结果表明，在缺氧条件下，PCa 细胞中 sTn 抗原的表达增加。此外，还发现ST6GalNAc1sTn 抗原合酶基因参与缺氧条件下 PCa 细胞的迁移-增殖二分法和耐药性。这项研究的结果将有助于在缺氧条件下开发新的 PCa 诊断标志物和药物靶点。