Abstract

The present research was performed to evaluate the phenotypic and genotypic properties of antibiotic resistance, virulence factor profiles and molecular typing of Staphylococcus aureus strains isolated from chicken meat. A total of 36 S. aureus strains were isolated from 300 raw chicken meat samples. Phenotypic pattern of antibiotic resistance was assessed by disk diffusion. Distribution of antibiotic resistance and virulence genes was evaluated by PCR. Molecular typing of isolates was performed by the ERIC-PCR. Considering the over than 80% similarity, 36 S. aureus isolates were classified in 9 different profiles with 43 to 100% similarities. S. aureus strains showed the highest incidence of resistance against penicillin (100%), tetracycline (91.66%), cephalothin (100%), ciprofloxacin (75%), erythromycin (75%), mupirocin (63.88%), clindamycin (61.11%) and trimethoprim/sulfamethoxazole (61.11%). The most commonly detected antibiotic resistance genes amid the S. aureus isolates were mecA (100%), tetK (80.55%), tetM (66.66%), aacA (6'')/aphD (2'') (61.11%), msrA (55.55%) and ermA (55.55%). Total distribution of etB, etA, tsst-1, clfA and coa virulence factors amongst the S. aureus strains was 61.11, 58.33, 13.88, 75 and 100%, respectively. Genetic cluster of bacteria affected the antibiotic resistance and virulence characters of S. aureus strains. S. aureus strains with the same ERIC-genetic cluster had similar antibiotic resistance and virulence characters which may show their similar origins. Presence of one or more virulence factors and antibiotic resistance genes amongst the resistant S. aureus strains signifies an important public health threat rendering the consumption of raw or undercooked chicken meat.

中文翻译：

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从生鸡肉中分离出的金黄色葡萄球菌的抗生素耐药性和毒力因子的分子分型、表型和基因型评估

摘要

本研究旨在评估从鸡肉中分离的金黄色葡萄球菌菌株的抗生素耐药性、毒力因子谱和分子分型的表型和基因型特性。从 300 个生鸡肉样品中分离出36 株金黄色葡萄球菌。通过纸片扩散评估抗生素耐药性的表型模式。通过PCR评估抗生素抗性和毒力基因的分布。通过 ERIC-PCR 对分离株进行分子分型。考虑到超过 80% 的相似性，36 株金黄色葡萄球菌分离株被分为 9 个不同的特征，相似性为 43% 至 100%。金黄色葡萄球菌菌株对青霉素（100%）、四环素（91.66%）、头孢噻吩（100%）、环丙沙星（75%）、红霉素（75%）、莫匹罗星（63.88%）、克林霉素（61.11%）和甲氧苄氨嘧啶/磺胺甲恶唑 (61.11%)。在金黄色葡萄球菌分离株中最常检测到的抗生素抗性基因是mecA (100%)、tetK (80.55%)、tetM (66.66%)、aacA (6'') / aphD (2'') (61.11%)、msrA (55.55%) 和ermA (55.55%)。etB , etA , tsst-1 , clfA和coa的总分布金黄色葡萄球菌菌株的毒力因子分别为 61.11、58.33、13.88、75 和 100%。细菌的遗传簇影响金黄色葡萄球菌菌株的抗生素抗性和毒力特性。具有相同 ERIC 基因簇的金黄色葡萄球菌菌株具有相似的抗生素抗性和毒力特征，这可能表明它们具有相似的起源。在抗性金黄色葡萄球菌菌株中存在一种或多种毒力因子和抗生素抗性基因意味着重要的公共健康威胁导致食用生的或未煮熟的鸡肉。