Mesenchymal stem cells with tissue repair capacity involve in regenerative medicine. MSCs can promote bone repair when employed with nano scaffolds/particles. Here, the MTT and Acridine Orange assay enabled the cytotoxic concentration of Zinc oxide nanoparticles and Polyurethane evaluation. Following culturing adipose tissue-derived MSCs, ADSCs' proliferation, growth, and osteogenic differentiation in the presence of PU with and without ZnO NPs is tracked by a series of biological assays, including Alkaline Phosphatase activity, Calcium deposition, alizarin red staining, RT-PCR, scanning electron microscope, and immunohistochemistry. The results showed boosted osteogenic differentiation of ADSCs in the presence of 1% PU scaffold and ZnO NPS and can thus apply as a new bone tissue engineering matrix. The expression level of Osteonectin, Osteocalcin, and Col1 increased in PU-ZnO 1% on the 7th and 14th days. There was an increase in the Runx2 gene expression on the 7th day of differentiation in PU-ZnO 1%, while it decreased on day 14th. In conclusion, Polyurethane nano scaffolds supported the MSCs’ growth and rapid osteogenic differentiation. The PU-ZnO helps not only with cellular adhesion and proliferation but also with osteogenic differentiation.

具有组织修复能力的间充质干细胞参与再生医学。当与纳米支架/颗粒一起使用时，MSCs 可以促进骨修复。在这里，MTT 和吖啶橙测定使氧化锌纳米颗粒的细胞毒性浓度和聚氨酯评估成为可能。在培养脂肪组织衍生的 MSCs 后，ADSCs 在 PU 存在和不存在 ZnO NPs 的情况下的增殖、生长和成骨分化通过一系列生物测定进行跟踪，包括碱性磷酸酶活性、钙沉积、茜素红染色、RT- PCR、扫描电子显微镜和免疫组织化学。结果表明，在 1% PU 支架和 ZnO NPS 存在的情况下，ADSC 的成骨分化得到促进，因此可以用作新的骨组织工程基质。骨连接蛋白的表达水平，在第 7 天和第 14 天，PU-ZnO 中的骨钙素和 Col1 增加了 1%。PU-ZnO 1% 分化第 7 天 Runx2 基因表达增加，第 14 天减少。总之，聚氨酯纳米支架支持 MSCs 的生长和快速成骨分化。PU-ZnO 不仅有助于细胞粘附和增殖，还有助于成骨分化。