Decalcified bone matrix (DBM) is a widely used alternative material for bone transplantation. In the DBM production process, an effective particle size and the highest utilization rate of raw materials can be achieved only through multiple high-speed circulating comminution. The rat posterolateral lumbar fusion model (PLF) is the most mature small animal model for the initial evaluation of the efficacy of graft materials for bone regeneration and spinal fusion. To evaluate the differences in the in vivo osteogenic effects of DBM pulverization through 1, 5, 9, and 14 high-speed cycles, sixty athymic rats were divided into six groups: single cycling crushing (CC1), 5 cycles of crushing (CC5), 9 cycles of crushing (CC9), 13 cycles of crushing (CC13), autogenous bone graft (ABG) and negative control (NC). Posterolateral lumbar fusion was performed. Six weeks after surgery, the bilateral lumbar fusion of athymic rats was evaluated through manual palpation, X-ray, micro-CT and histological sections. Rank data were tested by the rank-sum test, and nonparametric data were tested by the Kruskal‒Wallis H test. The manual palpation and X-ray results showed that the fusion rate did not significantly differ between the CC1, CC5, CC9, CC13 and ABG groups. However, cavities appeared in CC9 and CC13 on the micro-CT image. The bone mass (BV/TV) of CC1, CC5, CC9 and CC13 was better than that of the ABG group, while almost no osteogenesis was observed in the NC group. Histologically, there was no obvious difference between the four groups except that the CC9 group and CC13 group had more fibrous tissues in the new bone. In conclusion, DMB with different cycling crushing times has no obvious difference in fusion rate of PLF, but it is slightly better than the ABG group.

脱钙骨基质（DBM）是一种广泛使用的骨移植替代材料。DBM生产过程中，只有通过多次高速循环粉碎，才能达到有效粒度和最高的原料利用率。大鼠后外侧腰椎融合模型（PLF）是用于初步评价移植材料骨再生和脊柱融合功效的最成熟的小动物模型。为了评估 DBM 粉碎通过 1、5、9 和 14 个高速循环的体内成骨效果的差异，将 60 只无胸腺大鼠分为 6 组：单循环粉碎（CC1）、5 循环粉碎（CC5） ，9个粉碎周期（CC9），13个粉碎周期（CC13），自体骨移植（ABG）和阴性对照（NC）。进行了后外侧腰椎融合术。术后六周，通过徒手触诊、X线、显微CT和组织学切片评估无胸腺大鼠双侧腰椎融合情况。等级数据采用秩和检验，非参数数据采用Kruskal-Wallis H检验。手动触诊和X线检查结果显示CC1、CC5、CC9、CC13和ABG组之间融合率没有显着差异。然而，在显微CT图像上，CC9和CC13出现了空洞。CC1、CC5、CC9和CC13的骨量（BV/TV）优于ABG组，而NC组几乎没有观察到成骨。组织学上，除CC9组和CC13组新生骨中纤维组织较多外，四组间无明显差异。综上所述，不同循环破碎次数的DMB组PLF融合率无明显差异，但略优于ABG组。