Abstract

Identification of microorganisms using the method of time-of-flight mass spectrometry has been widely used in various fields of microbiology for more than a decade. Despite its rather narrow area of applicability compared to other areas of proteomic analysis, this technology has developed significantly, making it a tangible competitor to the traditional bacteriological method. Basically, this became possible due to the expansion of databases of comparative mass spectra of microorganisms and the capabilities of specialized programs. However, in addition to determining the taxonomic affiliation, in clinical or research microbiology, problems are often solved to determine the structure, properties, or number of specific proteins. The range of practical application of the results of proteomic analysis is very wide. In the field of medicine, this is the search for vaccines, markers of diseases of various etiologies, analysis of antibodies, and monitoring the effectiveness of therapeutic measures. In bacteriology or virology, the study of the properties of an infectious agent, as well as the pathogenesis of the disease it causes. In recent years, the range of possibilities of proteomic and bioinformatics has been continuously expanding, and the methods themselves have been improved. The combination of modern software systems with the capabilities of analytical equipment makes it possible to supplement the results of proteomic analysis with data from the fields of genomics, transcriptomics, and metabolomics. Such a trend in the future will lead to the possibility of a comprehensive laboratory assessment of any changes in the cell of the microorganism. In addition, this will contribute to the emergence of the most accurate and universal taxonomic systematization of known species, taking into account data on their genomic and proteomic composition. This review briefly describes the application of existing methods of proteomic analysis to the study of individual proteins or their mixtures.

中文翻译：

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微生物学中的蛋白质组学分析

摘要

十多年来，飞行时间质谱法鉴定微生物已广泛应用于微生物学的各个领域。尽管与蛋白质组学分析的其他领域相比，它的适用范围相当狭窄，但这项技术已经取得了长足的发展，使其成为传统细菌学方法的有力竞争者。基本上，由于微生物比较质谱数据库的扩展和专业程序的能力，这成为可能。然而，除了确定分类学隶属关系外，在临床或研究微生物学中，还经常解决确定特定蛋白质的结构、性质或数量的问题。蛋白质组学分析结果的实际应用范围非常广泛。在医药领域，这是寻找疫苗、各种病因疾病的标志物、抗体分析和监测治疗措施的有效性。在细菌学或病毒学中，研究传染原的特性及其引起的疾病的发病机制。近年来，蛋白质组学和生物信息学的可能性范围不断扩大，方法本身也得到了改进。现代软件系统与分析设备功能的结合使得用来自基因组学、转录组学和代谢组学领域的数据补充蛋白质组学分析的结果成为可能。未来的这种趋势将导致对微生物细胞的任何变化进行全面的实验室评估的可能性。此外，这将有助于对已知物种进行最准确和普遍的分类系统化，同时考虑到有关其基因组和蛋白质组学组成的数据。本综述简要描述了现有蛋白质组学分析方法在研究单个蛋白质或其混合物中的应用。