Abstract

Biobutanol is one of the types of biofuels produced using microbial synthesis (acetone–butanol–ethanol fermentation). It is possible to increase the level of accumulation of the target product due to metabolic changes in producer cells under the influence of stress factors. For this purpose, the effect of such stress factors as allopurinol, furfural, zinc, and oxygen, as well as glycerol as a nonspecific substrate on the functioning of Clostridium sp. cells, was studied. It was demonstrated that the butanol concentration was the highest (1.2 g/L) as compared with the control (0.6 g/L) when adding 0.5 g/L of furfural to the culture medium. The use of ZnSO₄ at a concentration of 0.001 g/L increased the butanol concentration to 1.1 g/L, and the highest butanol concentration (1.3 g/L) was obtained when using glucose and glycerol as carbon sources in a ratio of 1 : 3. The butanol concentration in the culture medium increased to 0.8 g/L with the addition of 0.025 g/L of allopurinol. The possibility of using the above-mentioned stress factors to increase the accumulation of the target product by influencing the metabolic pathways in microorganism cells is substantiated.

中文翻译：

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梭菌属 sp. 丁醇产量的增加。应激因素影响下的细胞

摘要

生物丁醇是使用微生物合成（丙酮-丁醇-乙醇发酵）生产的生物燃料类型之一。由于在应激因素的影响下生产细胞的代谢变化，有可能增加目标产物的积累水平。为此，别嘌醇、糠醛、锌和氧气等应激因子以及甘油作为非特异性底物对梭状芽孢杆菌功能的影响。细胞，进行了研究。结果表明，当向培养基中添加 0.5 g/L 糠醛时，与对照（0.6 g/L）相比，丁醇浓度最高（1.2 g/L）。_{ZnSO 4}的用途在浓度为0.001 g/L时，丁醇浓度增加到1.1 g/L，当以葡萄糖和甘油为碳源，比例为1:3时，丁醇浓度最高（1.3 g/L）。添加 0.025 g/L 别嘌呤醇后，培养基中的浓度增加到 0.8 g/L。证实了利用上述应激因子通过影响微生物细胞中的代谢途径来增加目标产物积累的可能性。