Background

Biofilm formation on biomedical devices is a prevalent problem that can result in several complications and is responsible for over 80% of all clinical infections. Successful treatment of biofilms requires a 1500-fold increase in antibiotic concentration, which can lead to toxicity and antibiotic resistance. Therefore, biofilm growth and infection in biomedical devices are significant concerns, and their prevention is a crucial medical challenge.

Objectives

To prevent biofilm infection by modifying the surface properties of medical devices, an oleamide–polydimethylsiloxane (PDMS) copolymer was synthesized to demonstrate anti-adhesion to bacteria and anti-biofilm activity. Catheter coatings for biomedical device applications were evaluated by blood toxicity.

Results

We synthesized an oleamide–PDMS copolymer (OPC) and evaluated its anti-adhesion and anti-biofilm activities against the gram-negative bacterium Escherichia coli (E. coli) and the gram-positive bacterium Staphylococcus aureus (S. aureus). The OPC films inhibited the growth of biofilms by inhibiting early adhesion of bacteria. As the oleamide content increased, the ability of the OPC films to inhibit E. coli and S. aureus surface adhesion also increased. In addition, the biofilm formation ability of both E. coli and S. aureus was significantly inhibited at oleamide contents of 2.5 wt% and 5 wt% in the OPC films, respectively. OPC films were applied to the catheter using a simple dip-coating method, and a low hemolytic capacity was confirmed by hemolysis analysis.

Conclusion

The anti-adhesion ability of OPC enables them to prevent biofilm growth and infection. Furthermore, they can be applied in medical devices owing to their high biocompatibility and poor hemolytic properties.

中文翻译：

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油酰胺-PDMS 共聚物，用于涂覆具有抗粘连和抗生物膜特性的导尿管

背景

生物医学设备上的生物膜形成是一个普遍存在的问题，可能导致多种并发症，并导致 80% 以上的临床感染。成功治疗生物膜需要将抗生素浓度增加 1500 倍，这可能会导致毒性和抗生素耐药性。因此，生物医学设备中的生物膜生长和感染是一个重大问题，其预防是一个至关重要的医学挑战。

目标

为了通过改变医疗器械的表面特性来防止生物膜感染，合成了油酰胺-聚二甲基硅氧烷（PDMS）共聚物，以证明其抗细菌粘附和抗生物膜活性。通过血液毒性评估用于生物医学设备应用的导管涂层。

结果

我们合成了一种油酰胺-PDMS 共聚物 (OPC)，并评估了其对革兰氏阴性菌大肠杆菌( E. coli ) 和革兰氏阳性菌金黄色葡萄球菌( S. aureus ) 的抗粘附和抗生物膜活性。OPC膜通过抑制细菌的早期粘附来抑制生物膜的生长。随着油酰胺含量的增加，OPC膜抑制大肠杆菌和金黄色葡萄球菌表面粘附的能力也增强。此外，大肠杆菌和金黄色葡萄球菌的生物膜形成能力OPC 薄膜中油酰胺含量分别为 2.5 wt% 和 5 wt% 时显着抑制。使用简单的浸涂方法将 OPC 薄膜涂覆到导管上，并通过溶血分析证实其溶血能力较低。

结论

OPC 的抗粘附能力使其能够防止生物膜生长和感染。此外，由于其高生物相容性和较差的溶血特性，它们可以应用于医疗器械。