Abstract—In this study, the complete mitochondrial genome (mt genome) of Triosteum pinnatifidum was investigated for the first time. The mt genome was consisted of 803 609 bp, comprised of 62 genes including 19 tRNA genes, 3 rRNA genes and 40 protein-coding genes. We detected protein-coding genes and codon usage, RNA editing sites and repeat sequences. The protein-coding genes substitution rates, DNA segments migration, comparison of six species genomic features were generated. Phylogenetic analysis of 32 species was also taken. A total of 463 RNA editing sites were found in 40 protein-coding genes. Being slightly positive, the GC-skew and AT-skew are 0.0060 and 0.0004, respectively. Most of PCGs have Ka/Ks ratio less than 1, indicating the existence of purifying or negative selection in these genes. This is the first report mt genome in the family Caprifoliaceae and could provide a useful foundation for evolutionary analysis, molecular biology and taxonomy in genus Triosteum and other higher plants.

摘要：本研究获得了Triosteum pinnatifidum的完整线粒体基因组（mt 基因组）首次被调查。mt基因组由803 609 bp组成，由62个基因组成，其中包括19个tRNA基因、3个rRNA基因和40个蛋白质编码基因。我们检测了蛋白质编码基因和密码子使用、RNA 编辑位点和重复序列。生成了蛋白质编码基因替换率、DNA 片段迁移、六个物种基因组特征的比较。还对 32 个物种进行了系统发育分析。在40个蛋白质编码基因中总共发现了463个RNA编辑位点。GC 偏斜和 AT 偏斜略为正，分别为 0.0060 和 0.0004。大多数PCG的Ka/Ks比值小于1，表明这些基因中存在纯化或负选择。这是忍冬科 mt 基因组的第一份报告，可以为进化分析提供有用的基础，三层板等高等植物。