Konjac glucomannan (KGM) has been reported to prevent high-fat diet-induced obesity, and we study investigated whether dietary supplementation with KGM can prevent obesity by increasing energy expenditure in inguinal white adipose tissue (iWAT) of high-fat diet (HF) -fed mice. Weaned mice fed the control diet (Con), HF, or HF plus KGM (8%, w/w, HFK) were divided into three groups. The results showed that 10-week supplementation with KGM significantly reduced partial adipose tissue weight and body weight, and improved glucose tolerance. Compared to the HF group, plasma lipid concentrations in the HFK group were greatly decreased to the control level. Moreover, transcriptomic research has shown that genes that are mainly associated with energy and lipid metabolism are significantly altered in iWAT. Mechanistically, KGM stimulated thermogenesis by promoting the expression of uncoupling protein-1 (UCP1) and the β3-adrenergic receptor (ADR3β). Taken together, our results suggest that dietary supplementation with konjac glucomannan can effectively alleviate obesity induced by a high-fat diet by activating ADR3β-mediated iWAT thermogenesis.

Graphical abstract

Dietary supplementation with KGM can effectively alleviate high fat diet- induced obesity mice by via activating ADR3β-mediated thermogenesis of iWAT.

中文翻译：

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魔芋葡甘露聚糖通过增强小鼠腹股沟白色脂肪组织中β-肾上腺素能介导的生热作用来减轻高脂饮食喂养的肥胖

据报道，魔芋葡甘聚糖 (KGM) 可以预防高脂饮食引起的肥胖，我们研究了膳食补充剂是否可以通过增加高脂饮食 (HF) 腹股沟白色脂肪组织 (iWAT) 的能量消耗来预防肥胖。 - 喂老鼠。喂食对照饮食 (Con)、HF 或 HF 加 KGM（8%，w/w，HFK）的断奶小鼠被分为三组。结果显示，补充KGM 10周可显着降低部分脂肪组织重量和体重，并改善糖耐量。与HF组相比，HFK组的血浆脂质浓度大大降低至对照水平。此外，转录组学研究表明，主要与能量和脂质代谢相关的基因在 iWAT 中发生了显着改变。从机制上讲，KGM 通过促进解偶联蛋白 1 (UCP1) 和 β3 肾上腺素受体 (ADR3β) 的表达来刺激产热。综上所述，我们的结果表明，膳食补充魔芋葡甘露聚糖可以通过激活 ADR3β 介导的 iWAT 生热作用，有效缓解高脂饮食引起的肥胖。

图形概要

膳食补充 KGM 可以通过激活 ADR3β 介导的 iWAT 生热作用，有效缓解高脂饮食诱导的肥胖小鼠。