Background

Stomach adenocarcinoma (STAD) is a leading contributor of cancer death and severely endangers human health worldwide. MicroRNA (miRNA) has been validated to involve in the pathogenesis of STAD. However, the potency of miR-365b-3p in STAD remains to be studied.

Objective

The goal of this work is to illuminate the function and mechanism of miR-365b-3p in STAD progression. The qPCR assay was conducted to identify the expression level of miR-365b-3p in STAD. The effects of miR-365b-3p on cell proliferation, apoptosis and angiogenesis were estimated with CCK-8, colony formation, western blot, tube formation and wound healing assays. Molecular mechanism was explored by RIP and luciferase reporter assays.

Results

Low expression of miR-365b-3p in STAD was provoked by epigenetic modification. miR-365b-3p acted as a cancer suppressor in the development of STAD by inhibiting cell growth and angiogenesis. Functionally, STC1 was a direct effector for miR-365b-3p. More than that, miR-365b-3p was sponged by HCG18 and mediated the promoting role of HCG18 in STAD progression.

Conclusion

This study unraveled that miR-365b-3p exerted the inhibitory impacts on cell proliferation and angiogenesis of STAD through targeting STC1 and interacting with HCG18 for the first time, which broadened our understanding of STAD etiology and contributed to the treatment of STAD.

中文翻译：

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HCG18吸附的miR-365b-3p通过下调STC1抑制胃腺癌的增殖和血管生成

背景

胃腺癌（STAD）是癌症死亡的主要原因，严重危害全世界人类健康。MicroRNA (miRNA) 已被证实参与 STAD 的发病机制。然而，miR-365b-3p 在 STAD 中的效力仍有待研究。

客观的

这项工作的目的是阐明 miR-365b-3p 在 STAD 进展中的功能和机制。进行qPCR测定来鉴定STAD中miR-365b-3p的表达水平。通过 CCK-8、集落形成、蛋白质印迹、管形成和伤口愈合测定评估 miR-365b-3p 对细胞增殖、凋亡和血管生成的影响。通过 RIP 和荧光素酶报告基因检测探索分子机制。

结果

STAD 中 miR-365b-3p 的低表达是由表观遗传修饰引起的。miR-365b-3p 通过抑制细胞生长和血管生成，在 STAD 的发展中充当癌症抑制剂。从功能上来说，STC1 是 miR-365b-3p 的直接效应子。更重要的是，miR-365b-3p 被 HCG18 吸收并介导 HCG18 在 STAD 进展中的促进作用。

结论

该研究首次揭示了miR-365b-3p通过靶向STC1并与HCG18相互作用对STAD细胞增殖和血管生成产生抑制作用，拓宽了我们对STAD病因的认识，为STAD的治疗做出了贡献。