Sampling surveys of Angelica acutiloba and A. acutiloba var. iwatensis, which are medicinal plants endemic to Japan, were conducted in the Chubu region in the central area of the main island of Japan. A. acutiloba grows in riverbeds in mountainous areas, while A. acutiloba. var. iwatensis grows on slopes near mountain ridges at 1000 m above sea level or on constantly collapsing rocky slopes and bare fields on developed land along asphalt roads in valleys of mountainous areas. Specimens of two wild Angelica species collected in this region were examined for maternal lineage by DNA polymorphism analysis of the atpF–atpA region for chloroplast DNA using direct sequencing and genomic component analysis by genome-wide SNP using MIG-seq. In this study area, while all A. acutiloba populations were monophyletic in both maternal and ancestral lineages, A. acutiloba var. iwatensis were genetically heterogeneous due to being composed of three maternal and three ancestral lineages to various degrees. In addition, a natural hybrid population with maternal lineage presumed to be A. acutiloba and paternal lineage A. acutiloba var. iwatensis was also found. In the present study, we report that the combined method of atpF–atpA and MIG-seq analyses is a useful tool for determining the population genetic structure of two wild Angelica species and for identifying hybrids.

当归和当归变种的抽样调查 岩藤是日本特有的药用植物，研究在日本本岛中部地区的中部地区进行。A. acutiloba生长在山区的河床，而A. acutiloba. 变种。岩瓦藤生长于海拔1000米的山脊附近的山坡上，或山区山谷中柏油路沿线的已开发土地上不断崩塌的岩石山坡和裸露的田野上。使用直接测序对叶绿体DNA 的atp F- atp A 区域进行 DNA 多态性分析，并使用 MIG-seq 通过全基因组 SNP 进行基因组成分分析，对在该区域收集的两种野生当归物种的样本进行母系谱系检查。在该研究区域，虽然所有A. acutiloba种群在母系和祖先谱系中都是单系的，但A. acutiloba var . iwatensis因不同程度地由三个母系和三个祖先谱系组成而具有遗传异质性。此外，一个自然杂交种群的母系推测为A. acutiloba，父系系为A. acutiloba var 。还发现了iwatensis 。在本研究中，我们报告说，atp F- atp A 和 MIG-seq 分析的组合方法是确定两种野生当归物种的群体遗传结构和鉴定杂种的有用工具。