Solena amplexicaulis (Lam.) Gandhi is a creeping cucurbit with high medicinal values in terms of its therapeutic uses. Traditionally, this plant species is used as a folklore medicine for the treatment of various disorders like spermatorrhea, urinary retention, jaundice, and cardiovascular disorders. Owing to high medicinal importance, it is overexploited, which has resulted in decrease in its population size in the wild. Furthermore, its natural propagation through root tubers and seeds is limited. In view of this constraint, the present study of in vitro micropropagation was chosen as an alternative for its propagation and conservation. Murashige and Skoog (MS) media amended with plant growth regulators like 6-benzylaminopurine (BAP) and thidiazuron (TDZ) independently and with a combination of indole-3-acetic acid (IAA) were tested for in vitro shoot induction by using nodal explants. The highest number of shoots (32.84±0.02) per explant was achieved on Murashige and Skoog (MS) medium with 1.0 mg L^-1 TDZ and 1.5 mg L^-1 IAA. The induced shoots were elongated with a maximum shoot length of 10.25±0.01 cm on MS medium augmented with 2.0 mg L^-1 gibberellin (GA₃) and 1.5 mg L^-1 TDZ. Rooting of in vitro raised shoots was attained on half-strength MS medium fortified with 1.0 mg L^-1 indole-3-butyric acid (IBA) and 2.0% sucrose. The hardening of resultant complete plantlets was carried out in plastic cups by using a 2:1 ratio of sterile black soil and sand; initially, they were kept in greenhouse conditions and subsequently shifted to natural field conditions with 78% of survival rate. The genetic fidelity of tissue-cultured plants was confirmed by ISSR (inter simple sequence repeats) and SCoT (starting codon targeted) primer-based analysis.

Solena amxicaulis (Lam.) Gandhi 是一种匍匐葫芦科植物，在治疗用途方面具有很高的药用价值。传统上，这种植物被用作民间医学，用于治疗遗精、尿潴留、黄疸和心血管疾病等各种疾病。由于具有很高的药用价值，它被过度开发，导致其野生种群数量减少。此外，其通过块根和种子的自然繁殖是有限的。鉴于这一限制，目前的体外研究选择微繁殖作为其繁殖和保存的替代方案。Murashige 和 Skoog (MS) 培养基分别用 6-苄氨基嘌呤 (BAP) 和噻苯隆 (TDZ) 等植物生长调节剂以及与吲哚-3-乙酸 (IAA) 的组合进行了改良，并通过使用节点外植体进行了体外芽诱导测试。^{在含有1.0 mg L -1} TDZ 和1.5 mg L ^-1 IAA的Murashige 和Skoog (MS) 培养基上获得每个外植体的最高芽数(32.84±0.02) 。在添加有2.0 mg L ^-1赤霉素(GA ₃ )和1.5 mg L ^-1 TDZ的MS培养基上诱导的芽被拉长，最大芽长度为10.25±0.01 cm。体外生根^{在用 1.0 mg L -1}吲哚-3-丁酸 (IBA) 和 2.0% 蔗糖强化的半强度 MS 培养基上获得了凸起的芽。将所得完整苗在塑料杯中使用2:1比例的无菌黑土和沙子进行硬化；最初，它们被饲养在温室条件下，随后转移到自然田间条件下，成活率达到 78%。组织培养植物的遗传保真度通过 ISSR（简单序列重复间）和 SCoT（起始密码子靶向）引物分析得到证实。