Biofertilization is a technique that uses plant and animal wastes to add organic matter and nutrients to the soil. It can also use microorganisms that can metabolize these by-products to facilitate their absorption by the plant roots. In this study, we tested the effects of rhizospheric bacteria inoculation (T₁), a combination of rhizospheric bacteria with liquid fertilizer (T₂) and uncombined liquid fertilizer (T₃), on the growth, nutritional content, root tissue, and root cells of lettuce plants. The results showed significant positive differences in all treatments compared to control plants, in terms of morphological, nutritional, and productivity parameters. The combination of rhizospheric bacteria with liquid SEFEL fertilizer (T₂) yielded the best results, showing increased fresh and dry weight, and diameter. There were no differences between treatments for nutritional content, but each treatment outperformed the control by more than 700% for all macronutrients. The best result was phosphorus content for T₁, with 1272.22% more than control. Regarding root structure and ultrastructure, there was no variation in tissue organization compared to control plants, but increases in root hairs (T₁), development of transfer cells (T₂), and secondary growth (T₃) were observed. Additionally, colonization of roots by rhizospheric bacteria was confirmed in all three treatments. In conclusion, this study suggests that inoculating with rhizospheric bacteria is a viable and environmentally friendly biofertilization for lettuce plants.

生物施肥是一种利用植物和动物废物向土壤添加有机物和养分的技术。它还可以利用能够代谢这些副产物的微生物来促进它们被植物根部吸收。_{在本研究中，我们测试了根际细菌接种（T 1 ）、根际细菌与液体肥料（T 2}）和未结合液体肥料（T ₃ ）的组合对生长、营养成分、根组织和根的影响。生菜植物的细胞。结果显示，与对照植物相比，所有处理在形态、营养和生产力参数方面均存在显着的积极差异。根际细菌与液体 SEFEL 肥料的组合（T ₂）产生了最好的结果，显示出增加的鲜重和干重以及直径。各处理之间的营养成分没有差异，但每种处理的所有常量营养素均优于对照 700% 以上。最好的结果是T ₁的磷含量，比对照高1272.22%。关于根结构和超微结构，与对照植物相比，组织组织没有变化，但根毛（T ₁）、转移细胞发育（T ₂）和次生生长（T ₃）被观察到。此外，在所有三种处理中都证实了根际细菌对根的定植。总之，这项研究表明，接种根际细菌是一种可行且环保的生菜生物施肥方法。