Purpose

Silver nanoparticles have been found to exhibit various bioactivities like antibacterial, anticancer, antifungal, and enzyme-mimicking properties and can be synthesized via chemical and biological methods.

Aim

This study compares the significant characteristics of chemically and biologically synthesized silver nanoparticles with sodium borohydride and Trigonella foenum-graecum leaf extract as reducing and stabilizing agents, respectively.

Methods

U.V. spectroscopy, FTIR (Fourier transform infrared) spectroscopy, DSC-TGA (Differential scanning calorimetry-Thermalgravimetric analysis), XPS (X-ray photoelectron spectrometer), and SEM (Scanning electron microscopy) were employed to characterize the nanoparticles. The antioxidant activity via FRAP assay, the nanoparticles’ cytotoxicity, and wound healing ability using MTT and scratch assay were performed.

Results

The absorption maxima were observed at 425 nm for green synthesized (GNP) and 390 nm for chemically synthesized silver nanoparticles (CNP). FTIR assignment and diffraction peaks at respective 2θ values confirmed the formation of AgNPs. The peak at 978°C in the thermogram proved the presence of silver in the biological method, and the first weight loss (42.48%) at 324.51°C for CNP exemplified the decomposition. The lower concentration of silver evidenced by XPS in GNP will benefit the toxicity-free biological effects induced by AgNPs. The presence of spherical-shaped GNP with better cell migration confirms faster wound healing with negligible toxicity compared to the multivariate-shaped CNP.

Conclusion

Although the chemically synthesized silver nanoparticle exhibited good antioxidant activity, the biogenic nanoparticle showed better antimicrobial and wound-healing potential with lesser cytotoxicity on fibroblast cells relating to the size and shape of the nanoparticles synthesized. Therefore, further studies on formulations using biogenic silver nanoparticles are worth pondering.

中文翻译：

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用胡芦巴叶合成的银纳米粒子比化学合成的纳米粒子具有生物学效力

目的

银纳米颗粒已被发现具有多种生物活性，如抗菌、抗癌、抗真菌和模拟酶特性，并且可以通过化学和生物方法合成。

目的

本研究比较了分别以硼氢化钠和葫芦巴叶提取物作为还原剂和稳定剂的化学和生物合成银纳米粒子的显着特性。

方法

采用紫外光谱、FTIR（傅里叶变换红外）光谱、DSC-TGA（差示扫描量热法-热重分析）、XPS（X射线光电子能谱仪）和SEM（扫描电子显微镜）对纳米粒子进行表征。通过 FRAP 测定法测定抗氧化活性，使用 MTT 和划痕测定法测定纳米颗粒的细胞毒性和伤口愈合能力。

结果

绿色合成 (GNP) 的最大吸收波长为 425 nm，化学合成的银纳米颗粒 (CNP) 的最大吸收波长为 390 nm。FTIR 分配和各自 2θ 值处的衍射峰证实了 AgNP 的形成。热分析图中 978°C 处的峰值证明了生物方法中银的存在，CNP 在 324.51°C 处的首次失重（42.48%）说明了分解。XPS 证明 GNP 中银的浓度较低，有利于 AgNP 诱导的无毒生物效应。与多元形状的 CNP 相比，具有更好细胞迁移能力的球形 GNP 的存在证实了伤口愈合更快，且毒性可忽略不计。

结论

尽管化学合成的银纳米颗粒表现出良好的抗氧化活性，但生物纳米颗粒表现出更好的抗菌和伤口愈合潜力，并且对成纤维细胞的细胞毒性较小，这与合成纳米颗粒的大小和形状有关。因此，使用生物银纳米粒子的配方的进一步研究值得思考。