Background

Many studies have shown that abnormal circular RNA (circRNA) expression is associated with the malignant progression of breast cancer (BC), but the role of circ_0000732 in BC progression remains unclear.

Methods

The expression of circ_0000732, microRNA (miR)-1253 and collagen XI alpha 1 (COL11A1) was measured by quantitative real-time PCR. Cell proliferation, migration, invasion and stemness were assessed by cell counting kit 8 assay, Edu assay, transwell assay and sphere formation assay. Western blot analysis was used to determine protein expression. Dual-luciferase reporter assay was performed to assess the interaction between miR-1253 and circ_0000732 or COL11A1. The effect of circ_0000732 on BC tumor growth was confirmed by animal experiments.

Results

Circ_0000732 was overexpressed in BC tissues and cells, and its knockdown suppressed BC cell proliferation, metastasis and stemness. MiR-1253 could be sponged by circ_0000732, and anti-miR-1253 overturned the effects of circ_0000732 knockdown on BC cell progression. COL11A1 was targeted by miR-1253, and miR-1253 inhibited BC cell progression by targeting COL11A1. Circ_0000732 could sponge miR-1253 to upregulate COL11A1. Also, interference of circ_0000732 decreased BC tumor growth by miR-1253/COL11A1 pathway.

Conclusion

Our data showed that circ_0000732 might be a potential target for BC treatment, which could enhance BC malignant phenotype through miR-1253/COL11A1 axis.

中文翻译：

---

Circ_0000732通过调节miR-1253介导COL11A1促进乳腺癌细胞增殖、转移和干性

背景

许多研究表明，异常的环状RNA（circRNA）表达与乳腺癌（BC）的恶性进展相关，但circ_0000732在BC进展中的作用仍不清楚。

方法

通过定量实时 PCR 测量 circ_0000732、microRNA (miR)-1253 和胶原蛋白 XI α 1 (COL11A1) 的表达。通过细胞计数试剂盒8测定、Edu测定、transwell测定和球体形成测定评估细胞增殖、迁移、侵袭和干性。使用蛋白质印迹分析来确定蛋白质表达。进行双荧光素酶报告基因测定以评估 miR-1253 和 circ_0000732 或 COL11A1 之间的相互作用。circ_0000732对BC肿瘤生长的影响通过动物实验得到证实。

结果

Circ_0000732在BC组织和细胞中过表达，其敲低可抑制BC细胞增殖、转移和干性。miR-1253 可以被 circ_0000732 吸收，抗 miR-1253 推翻了 circ_0000732 敲低对 BC 细胞进展的影响。COL11A1 是 miR-1253 的靶点，而 miR-1253 通过靶向 COL11A1 抑制 BC 细胞进展。Circ_0000732 可以海绵 miR-1253 上调 COL11A1。此外，circ_0000732 的干扰通过 miR-1253/COL11A1 途径降低了 BC 肿瘤的生长。

结论

我们的数据表明，circ_0000732可能是BC治疗的潜在靶点，它可以通过miR-1253/COL11A1轴增强BC恶性表型。