Background

Acute pancreatitis (AP) is an abdominal disease mainly characterized by oxidative stress and inflammation of the pancreas. Studies have reported that scutellarein has antioxidant and anti-inflammatory properties.

Objectives

To explore whether scutellarein is protective against AP induced by L-arginine.

Results

Scutellarein administration mitigated inflammatory infiltration, edema and acinar cells necrosis of pancreatic tissue, and dramatically reduced the elevated serum levels of amylase in AP mice. Scutellarein inhibited the activity of MPO, MDA and ROS, and inhibited VEGF expression and the phosphorylation of NF-kBp65 in pancreatic tissues of AP mice, and reduced the production of pro-inflammatory cytokines Interleukin 6 (IL-6), Tumor necrosis factor α (TNF-α) and IL-1β. In addition, through AAV-VEGF upregulated the expression of VEGF in AP mice, the protective effect of scutellarein on AP mice were reversed.

Conclusion

Scutellarein treatment alleviated pancreas injury of AP mice by inhibiting oxidative stress and inflammatory response via downregulating VEGF expression.

中文翻译：

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黄芩素通过调节 VEGF 表达改善 L-精氨酸诱导的小鼠急性胰腺炎炎症和氧化应激

背景

急性胰腺炎（AP）是一种以胰腺氧化应激和炎症为主要特征的腹部疾病。研究表明，灯盏花素具有抗氧化和抗炎特性。

目标

探讨灯盏乙素是否对 L-精氨酸诱导的 AP 具有保护作用。

结果

黄芩素给药减轻了AP小鼠胰腺组织的炎症浸润、水肿和腺泡细胞坏死，并显着降低了升高的血清淀粉酶水平。黄芩素抑制AP小鼠胰腺组织中MPO、MDA和ROS活性，抑制VEGF表达和NF-kBp65磷酸化，减少促炎细胞因子白细胞介素6（IL-6）、肿瘤坏死因子α的产生(TNF-α) 和 IL-1β。此外，通过AAV-VEGF上调AP小鼠中VEGF的表达，逆转了灯盏乙素对AP小鼠的保护作用。

结论

黄芩素治疗通过下调 VEGF 表达抑制氧化应激和炎症反应，从而减轻 AP 小鼠的胰腺损伤。