Microplastics have been detected in a variety of aquatic ecosystems, and the combined effect of microplastics and chemical pollutants has become a matter of increasing concern. We conducted a 12-d co-exposure test of anthracene and spherical or fragmented polyethylene microplastics (size 200 µm) on Java medaka (Oryzias javanicus). The accumulation of anthracene in Java medaka muscle reached a plateau on day 5 in all anthracene exposure groups, and no significant differences were detected among the groups (ANT, 20.4 ± 5.5; ANT + SPPE-MP, 24.7 ± 2.7; ANT + FRPE-MP, 24.6 ± 4.7 µg/g). However, co-exposure to anthracene and spherical or fragmented polyethylene microplastics increased the duration of slow swimming in a swimming behavior test (control, 4.1 ± 1.4; ANT, 5.2 ± 2.8; ANT + SPPE-MP, 12.4 ± 3.7; ANT + FRPE-MP, 17.4 ± 5.1 min/30 min), and co-exposure to anthracene and fragmented polyethylene microplastics induced higher cytochrome P4501A monooxygenase (CYP1A) expression in Java medaka livers than the other anthracene exposure groups (ANT, 189 ± 74; ANT + SPPE-MP, 203 ± 75; ANT + FRPE-MP 272 ± 36% of control). Polyethylene microplastics appear to be weak vectors of anthracene at the size tested (200 µm), and the effect of shape (spherical or fragmented) on the vector effect was small. However, the presence of polyethylene microplastics could affect the swimming behavior and CYP1A expression in Java medaka.

多种水生生态系统中均检测到了微塑料，微塑料与化学污染物的综合影响已成为人们日益关注的问题。我们对爪哇青鳉 ( Oryzias javanicus )进行了蒽和球形或碎片聚乙烯微塑料（尺寸 200 µm）12 天的共同暴露测试。所有蒽暴露组中爪哇青鳉肌肉中蒽的积累在第 5 天达到平台，各组之间没有检测到显着差异（ANT，20.4 ± 5.5；ANT + SPPE-MP，24.7 ± 2.7；ANT + FRPE- MP，24.6 ± 4.7 微克/克）。然而，在游泳行为测试中，同时接触蒽和球形或碎片聚乙烯微塑料会增加缓慢游泳的持续时间（对照，4.1±1.4；ANT，5.2±2.8；ANT + SPPE-MP，12.4±3.7；ANT + FRPE） -MP，17.4 ± 5.1 分钟/30 分钟）以及同时暴露于蒽和碎片化聚乙烯微塑料可诱导爪哇青鳉肝脏中细胞色素 P4501A 单加氧酶 ( CYP1A )的表达高于其他蒽暴露组（ANT，189 ± 74；ANT + SPPE-MP，203 ± 75；ANT + FRPE-MP 272 ± 36% 对照）。在测试的尺寸（200微米）下，聚乙烯微塑料似乎是蒽的弱载体，并且形状（球形或碎片）对载体效应的影响很小。然而，聚乙烯微塑料的存在可能会影响爪哇青鳉的游泳行为和CYP1A表达。