Long noncoding RNAs (lncRNAs) have recently been identified as crucial biomarkers of papillary thyroid cancer (PTC). In this study, we aimed to investigate the biological function and potential clinical role of lncRNA ENST00000494165 in PTC. From January 2019 to December 2019, 226 PTC patients who underwent preoperative thyroid US-fine needle aspiration biopsy(US-FNAB) and confirmed by postoperative pathology were enrolled at our hospital. Thyroid tissues were collected from FNAB samples and stored in the refrigerator at –80°C. qRT-PCR (quantitative reverse transcriptase-polymerase chain reaction) analysis was performed to detect the relative expression level of ENST00000494165. CCK-8 (Cell Counting Kit-8) and colony formation assay were performed to detect the cell proliferation ability. Cell migration and invasion abilities were evaluated by transwell and scratch assay. We also evaluated the relation between the expression level of ENST00000494165 and the clinicopathological features of PTC. Functional assays demonstrated that the cell proliferation, migration and invasion abilities were all promoted in PTC cell lines when ENST00000494165 expression was overexpressed. The expression of ENST00000494165 in the lymph node metastasis group was significantly higher than that in non-lymph node metastasis group. High level expression of ENST00000494165 was significantly associated with lymph node metastasis of PTC (P < 0.001). The overexpression of ENST00000494165 promoted the progression, migration and invasion abilities of PTC and was significantly correlated with lymph node metastasis in PTC. ENST00000494165 could act as a possible promoter gene and a potential biomarker for PTC.

长非编码 RNA (lncRNA) 最近被确定为甲状腺乳头状癌 (PTC) 的重要生物标志物。在本研究中，我们旨在研究lncRNA ENST00000494165在PTC中的生物学功能和潜在的临床作用。2019年1月至2019年12月，我院入组226例术前接受甲状腺超声细针抽吸活检（US-FNAB）并经术后病理证实的PTC患者。从 FNAB 样本中收集甲状腺组织并储存在 –80°C 的冰箱中。进行qRT-PCR（定量逆转录酶-聚合酶链式反应）分析以检测ENST00000494165的相对表达水平。采用CCK-8(Cell Counting Kit-8)和集落形成实验检测细胞增殖能力。通过transwell和划痕实验评估细胞迁移和侵袭能力。我们还评估了 ENST00000494165 的表达水平与 PTC 临床病理特征之间的关系。功能分析表明，当ENST00000494165表达过表达时，PTC细胞系的细胞增殖、迁移和侵袭能力均得到促进。淋巴结转移组ENST00000494165的表达量显着高于非淋巴结转移组。ENST00000494165的高水平表达与PTC的淋巴结转移显着相关（当 ENST00000494165 表达过表达时，PTC 细胞系的迁移和侵袭能力均得到促进。淋巴结转移组ENST00000494165的表达量显着高于非淋巴结转移组。ENST00000494165的高水平表达与PTC的淋巴结转移显着相关（当ENST00000494165表达过表达时，PTC细胞系的迁移和侵袭能力均得到促进。淋巴结转移组ENST00000494165的表达量显着高于非淋巴结转移组。ENST00000494165的高水平表达与PTC的淋巴结转移显着相关（P < 0.001）。ENST00000494165的过表达促进了PTC的进展、迁移和侵袭能力，并与PTC的淋巴结转移显着相关。ENST00000494165 可以作为 PTC 的可能启动子基因和潜在生物标志物。