The intergenic spacer (IGS) of 5S ribosomal RNA genes (5S rDNA) (that are present in the genome of all living organisms) is characterized by a high variability, due to which it is a convenient and widely used object for clarifying the issues of genome evolution, population genetics, systematics, etc. The aim of this work was to study the IGS of 5S rDNA of the Antarctic pearlwort Colobanthus quitensis. The nucleotide sequence was determined by the methods of molecular genetic analysis, and molecular organization of the C. quitensis 5S rDNA IGS was studied. It was demonstrated that it contains the regulatory elements typical for other vascular plants. The existence of at least two classes of 5S rDNA repeats in the individual genome (that differ significantly in the length and nucleotide sequence of IGS) was established. In addition, based on differences in the length and nucleotide sequence, two subclasses of repeats with a long IGS and three subclasses with a short IGS were identified. A comparison of 5S rDNA IGS sequences in C. quitensis and Silene latifolia Poir. (a member of another section of the Caryophyllaceae family) demonstrated significant differences in the structure of a spacer region of 5S rRNA genes (except for its parts containing regulatory elements). In general, data obtained indicate a significant level of intragenomic polymorphism of C. quitensis 5S rDNA IGS.

5S核糖体RNA基因（5S rDNA）（存在于所有生物体的基因组中）的基因间间隔区（IGS）具有高度可变性的特点，因此它是一个方便且广泛使用的对象，用于澄清以下问题：基因组进化、群体遗传学、系统学等。本工作的目的是研究南极珍珠草Colobanthus quitensis 5S rDNA的IGS 。通过分子遗传学分析和C. quitensis的分子组织的方法确定了核苷酸序列研究了5S rDNA IGS。事实证明，它含有其他维管植物典型的调节元件。个体基因组中至少存在两类 5S rDNA 重复序列（IGS 的长度和核苷酸序列显着不同）。此外，根据长度和核苷酸序列的差异，鉴定了两个具有长IGS的重复亚类和三个具有短IGS的重复亚类。C. quitensis和Silene latifolia 5S rDNA IGS 序列的比较波伊尔。（石竹科另一部分的成员）在 5S rRNA 基因的间隔区结构中表现出显着差异（除了其含有调控元件的部分）。一般而言，获得的数据表明C. quitensis 5S rDNA IGS 具有显着水平的基因组内多态性。