Apple growth and development can be impacted by abiotic factors, including low temperature and dryness. Plant responses to stressors are heavily influenced by inducer of CBF expression (ICE) transcription factors (TFs). Malus baccata (L.) Borkh (M. baccata) was used in this investigation to isolate the MbICE3 new ICE gene. The molecular weight of MbICE3 was anticipated to be 56.47 kDa, and its open reading frame (ORF) had 519 amino acids (aa) and 1560 base pairs (bp). Subcellular localization revealed that MbICE3 was concentrated in the nucleus. Cold and drought stresses significantly affected the expression levels of MbICE3, and the first heterologous expression of MbICE3 in lettuce (Lactuca sativa L.) could significantly improve its tolerance to cold and drought stresses. In comparison to wild-type (WT) and unloaded lines (UL) lettuce, the transgenic plants had increased proline and chlorophyll concentrations as well as higher superoxide dismutase (SOD) and peroxidase (POD) activity when subjected to cold and drought. However, compared to WT and UL, transgenic lettuce had lower levels of malondialdehyde (MDA) and O₂⁻ content. Furthermore, it was found that under drought and low temperature stress, MbICE3 positively regulated the expression of LsCBFs genes. These results suggest that MbICE3 actively contributes to plant tolerance to cold and drought.

苹果的生长和发育可能会受到非生物因素的影响，包括低温和干燥。植物对应激源的反应很大程度上受到 CBF 表达诱导物 (ICE) 转录因子 (TF) 的影响。本研究中使用Malus baccata (L.) Borkh ( M. baccata ) 来分离MbICE3新 ICE 基因。MbICE3的分子量预计为56.47 kDa，其开放阅读框（ORF）有519个氨基酸（aa）和1560个碱基对（bp）。亚细胞定位显示MbICE3集中在细胞核中。寒冷和干旱胁迫显着影响MbICE3的表达水平，并且首次异源表达生菜（Lactuca sativa L.）中的MbICE3可以显着提高其对寒冷和干旱胁迫的耐受性。与野生型（WT）和无负载系（UL）生菜相比，转基因植物在遭受寒冷和干旱时具有更高的脯氨酸和叶绿素浓度以及更高的超氧化物歧化酶（SOD）和过氧化物酶（POD）活性。_{然而，与WT和UL相比，转基因生菜的丙二醛(MDA)和O 2} ^-含量水平较低。此外，还发现在干旱和低温胁迫下，MbICE3正向调节LsCBFs基因的表达。这些结果表明MbICE3积极促进植物对寒冷和干旱的耐受性。