Hedychium coronarium J. Koenig is a rhizomatous herb (Zingiberaceae family) and is well known for its uses in traditional systems of medicine for the treatment of various diseases. The plant has been over-exploited and enlisted as a threatened species in India. Thus, there is an urgent attention required for its conservation. Here, a callus-mediated in vitro plant regeneration protocol was developed using leaf sheath of H. coronarium. The optimal medium for callus induction (85.0%) and subsequent proliferation was found to be MS basal medium augmented with 3.0 mg L⁻¹ N⁶-benzyladenine (BA), 0.5 mg L⁻¹ α-naphthaleneacetic acid (NAA), and 3.0% sucrose, and gelled with 0.5% agar. Optimum callus-mediated shoot organogenesis (78.3%; ca. 11.4 shoots/0.5 g of callus) was obtained on MS medium supplemented with 3.0 mg L⁻¹ BA after 6 wk of culture. All the in vitro regenerated shoots were rooted (8.5 roots/shoot) successfully on plant growth regulator–free MS medium. About 90% plantlets were acclimatized on the planting tray filled with garden soil and sand (1:1). Transfer of these plants to larger pots containing garden soil and subsequent field transfer under full sun resulted in cent-percent survival. Monomorphic banding profile obtained using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers ascertained the clonal fidelity of the in vitro regenerated plants. Similarly, the biochemical fidelity of the in vitro regenerated plants vis-à-vis mother plant was ascertained by comparing the composition of essential oil through gas chromatography/mass spectrometry (GC/MS) analysis. Furthermore, the antioxidant activities estimated by DPPH and ABTS free radical scavenging assay and anticancer activities evaluated against two cell lines, i.e., MCF 7 and MDA-MB 231, also confirmed comparable effectiveness of in vitro regenerated plants to that of the mother plant. Thus, the study has the potential to provide a platform to achieve sustainability by using the in vitro regenerated H. coronarium in place of naturally available population.

Hedychium coronarium J. Koenig 是一种根茎草本植物（姜科），以其在传统医学系统中治疗各种疾病的用途而闻名。该植物在印度已被过度开发并列为受威胁物种。因此，对其保护刻不容缓。在这里，使用H. coronarium的叶鞘开发了愈伤组织介导的体外植物再生方案。愈伤组织诱导 (85.0%) 和随后增殖的最佳培养基是添加了 3.0 mg L ^-1 N ⁶ -苄基腺嘌呤 (BA)、0.5 mg L ^{-1的 MS 基础培养基。}α-萘乙酸 (NAA) 和 3.0% 蔗糖，并用 0.5% 琼脂胶凝。^{培养6周后，在补充有3.0 mg L -1} BA的MS培养基上获得最佳愈伤组织介导的芽器官发生（78.3%；约11.4芽/0.5 g愈伤组织） 。所有体外再生芽均在不含植物生长调节剂的 MS 培养基上成功生根（8.5 根/芽）。约90%的试管苗在装满园土和沙子（1:1）的种植盘上已适应。将这些植物转移到装有花园土壤的较大花盆中，然后在充足的阳光下进行田间转移，导致百分之一的成活率。使用随机扩增多态性 DNA (RAPD) 和间简单序列重复 (ISSR) 标记获得的单态带谱确定了克隆保真度体外再生植物。同样，通过气相色谱/质谱 (GC/MS) 分析比较精油的成分，确定了体外再生植物相对于母体植物的生化保真度。此外，通过DPPH和ABTS自由基清除测定估计的抗氧化活性以及针对两种细胞系(即MCF 7和MDA-MB 231)评估的抗癌活性也证实了体外再生植物与母体植物的相当的有效性。因此，该研究有可能提供一个平台，通过使用体外再生的冠状嗜血杆菌来实现可持续性代替自然可用的人口。