Abstract

Histone acetyltransferases of the CBP/p300 family are involved in transcriptional regulation and many biological processes (cell proliferation and differentiation, development, and regulation of the stress response and metabolism). Overexpression and knockdown of the nejire (nej) gene (codes for an ortholog of human CBP/p300 proteins) in various tissues (the fat body, intestine, and nervous system) and at various stages of the life cycle (throughout all developmental stages or in adulthood only) were tested for effect on lifespan in the fruit fly Drosophila melanogaster. The activation of nej exerted a positive or a negative effect on the lifespan, depending on the induction mode and the sex. A 6–15% greater lifespan was observed in females with conditional overexpression of nej in the intestine and constitutive overexpression of nej in the nervous system. A decrease (to 44%) or lack of significant changes in lifespan was detected in all other cases. observed. In addition, stress response genes (Sod1, Gadd45, Hsp27, Hsp68, and Hif1) were regulated by nej activation. nej knockdown caused a pronounced negative effect on the D. melanogaster lifespan in most variants of the experiment.

中文翻译：

---

果蝇寿命受外周组织和神经系统 nejire 基因表达的调节

摘要

CBP/p300 家族的组蛋白乙酰转移酶参与转录调节和许多生物过程（细胞增殖和分化、发育以及应激反应和代谢的调节）。nejire ( nej ) 基因（编码人类 CBP/p300 蛋白的直向同源物）在各种组织（脂肪体、肠道和神经系统）和生命周期的各个阶段（整个发育阶段或在果蝇果蝇中测试了对寿命的影响（仅在成年期）。nej的激活对寿命产生积极或消极影响，取决于诱导模式和性别。在肠道中条件性过度表达nej和神经系统中持续过度表达nej的女性中，观察到寿命延长了 6-15% 。在所有其他案例中均检测到寿命减少（至 44%）或没有显着变化。观察到的。此外，应激反应基因（Sod1、Gadd45、Hsp27、Hsp68和Hif1）受到nej激活的调节。nej敲除对黑腹果蝇产生了明显的负面影响大多数实验变体的寿命。