Background

Fucoidan, a polysaccharide derived from brown seaweed, has multiple biological properties, including antioxidant, antitumor, anticoagulant, and anti-inflammatory effects. However, there are few reports on their neuroprotective effects.

Objective

This study is about the neuroprotective effects of fucoidan against Aβ42-induced neuronal apoptosis in human neuroblastoma SH-SY5Y cells.

Methods

The protective effects of fucoidan against cell death by Aβ42 were measured with FDA/PI and Hoechst 33342 staining and immunoblotting about apoptosis-related proteins.

Results

In this study, fucoidan activated autophagy by regulating the expression of autophagy markers, significantly upregulating the Bcl-2/Bax ratio, and downregulating cleaved caspase-3/caspase-3. Moreover, fucoidan improved Aβ42-induced neuronal apoptosis by activating the phosphatidylinositol 3-kinase/protein kinase B signaling pathway and mitogen-activated protein kinase cascade in SH-SY5Y cells. Additionally, Hoechst 33342 staining showed that fucoidan diminished the level of the apoptotic nuclei in Aβ42-induced SH-SY5Ycells.

Conclusion

This study proposes fucoidan as a potential therapeutic candidate for AD treatment.

中文翻译：

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褐藻糖胶通过激活人神经母细胞瘤 SH-SY5Y 细胞中的 PI3K/Akt 信号通路和 MAPK 级联反应改善淀粉样蛋白-β 42 寡聚物诱导的神经元凋亡

背景

褐藻糖胶是一种从褐藻中提取的多糖，具有多种生物特性，包括抗氧化、抗肿瘤、抗凝血和抗炎作用。然而，关于其神经保护作用的报道却很少。

客观的

本研究是关于岩藻依聚糖对 Aβ42 诱导的人神经母细胞瘤 SH-SY5Y 细胞神经元凋亡的神经保护作用。

方法

通过 FDA/PI 和 Hoechst 33342 染色以及凋亡相关蛋白的免疫印迹测量岩藻依聚糖对 Aβ42 引起的细胞死亡的保护作用。

结果

在本研究中，岩藻依聚糖通过调节自噬标记物的表达、显着上调 Bcl-2/Bax 比率、下调 cleaved caspase-3/caspase-3 来激活自噬。此外，岩藻依聚糖通过激活 SH-SY5Y 细胞中的磷脂酰肌醇 3-激酶/蛋白激酶 B 信号通路和丝裂原激活的蛋白激酶级联，改善 Aβ42 诱导的神经元凋亡。此外，Hoechst 33342 染色显示岩藻依聚糖降低了 Aβ42 诱导的 SH-SY5Y 细胞中凋亡细胞核的水平。

结论

这项研究提出岩藻依聚糖作为 AD 治疗的潜在候选药物。