In this study, investigation of the effects of Quercetin on Bleomycin-induced pulmonary fibrosis and fibrosis-associated molecules miR-26b and miR-27b was aimed. Control group was given 10% saline on the 0th day, and saline was administered for 21 days starting from the 8th day. Group 2 was given 50 mg/kg Quercetin for 21 days starting from the 8th day. Group 3 was given 10 mg/kg Bleomycin Sulfate on day 0, and sacrificed on the 22nd and 29th day. Group 4 was given 10 mg/kg Bleomycin Sulfate on the 0th day, and was given 50 mg/kg Quercetin for 14 days, and 21 days starting from day 8. Lung tissues were examined using light and electron microscopic, immunohistochemical and molecular biological methods. Injury groups revealed impaired alveolar structure, collagen accumulation and increased inflammatory cells in interalveolar septum. Fibrotic response was decreased and the alveolar structure was improved with Quercetin treatment. α-SMA expressions were higher in the injury groups, but lower in the treatment groups compared to the injury groups. E-cadherin expressions were decreased in the injury groups and showed stronger immunoreactivity in the treatment groups compared to the injury groups. miR-26b and miR-27b expressions were lower in the injury groups than the control groups, and higher in the treatment groups than the injury groups. Quercetin can be considered as a new treatment agent in the idiopathic pulmonary fibrosis, since it increases the expression levels of miR-26b and miR-27b which decrease in fibrosis, and has therapeutic effects on the histopathological changes.

本研究旨在探讨槲皮素对博来霉素诱导的肺纤维化以及纤维化相关分子miR-26b和miR-27b的影响。对照组第0天给予10%生理盐水，第8天开始给予生理盐水21天。第2组从第8天开始给予50mg/kg槲皮素，持续21天。第3组在第0天给予10mg/kg硫酸博莱霉素，并在第22天和第29天处死。第4组第0天给予10 mg/kg硫酸博来霉素，第14天给予50 mg/kg槲皮素，第8天开始给予21天。采用光镜、电镜、免疫组化和分子生物学方法检查肺组织。损伤组显示肺泡结构受损、胶原蛋白积累和肺泡间隔炎症细胞增加。槲皮素治疗可降低纤维化反应并改善肺泡结构。与损伤组相比，损伤组的 α-SMA 表达较高，但治疗组的表达较低。与损伤组相比，损伤组中E-钙粘蛋白表达降低，并且治疗组中显示出更强的免疫反应性。损伤组中 miR-26b 和 miR-27b 的表达量低于对照组，治疗组中的表达量高于损伤组。槲皮素可以被认为是特发性肺纤维化的新治疗剂，因为它可以增加miR-26b和miR-27b的表达水平，从而减少纤维化，并对组织病理学变化具有治疗作用。