True homozygous pure lines are required for the development of new crop varieties. In conventional as well as molecular breeding strategies, it typically takes 7–9 generations to accomplish the appropriate level of homozygosity. On the contrary, haploids can have their chromosomes doubled in a single generation to create a true-breeding lineage. Over the period, researchers have developed several methods for haploids and doubled haploid induction, but these methods are only applicable to specific crop types. The discovery of the centromere-specific histone 3 variant (CENH3) and its manipulation is proving to be the most potent technique for haploid development. Recent advancements in this technology have shown that non-transgenic changes to CENH3 can also induce haploids. Point mutations in CENH3 that can be induced by chemical agents may lead to haploid induction when crossed with wild-type CENH3 plants. These plants with the CENH3 mutation are fully fertile when selfed, develop properly, and can be found in already-existing collections of mutagenized plants. The current review encompasses the recent studies undertaken to utilize the CENH3 manipulations strategy to develop various haploid plant crops with increased success rates. This review paper will provide a better insight into understanding the detailed mechanism of the CENH3-induced haploid induction process and help investigate the areas that need to be further explored.

开发新作物品种需要真正的纯合纯系。在传统和分子育种策略中，通常需要 7-9 代才能达到适当的纯合性水平。相反，单倍体的染色体可以在一代中加倍，以创建真正的育种谱系。在此期间，研究人员开发了几种单倍体和双单倍体诱导的方法，但这些方法仅适用于特定的作物类型。着丝粒特异性组蛋白 3 变体 (CENH3) 的发现及其操作被证明是单倍体发育最有效的技术。这项技术的最新进展表明，CENH3 的非转基因变化也可以诱导单倍体。当与野生型 CENH3 植物杂交时，化学试剂诱导的 CENH3 点突变可能会导致单倍体诱导。这些带有 CENH3 突变的植物在自交时完全可育，发育正常，并且可以在现有的诱变植物集合中找到。当前的综述涵盖了最近利用 CENH3 操作策略开发各种单倍体作物并提高成功率的研究。这篇综述论文将有助于更好地理解 CENH3 诱导的单倍体诱导过程的详细机制，并有助于研究需要进一步探索的领域。