The present study aimed to detect and eliminate Grapevine leafroll-associated viruses (GLRaVs) in commercially available Vitis varieties in order to produce appropriate clones for certification. A total of nine commercially available grapevine (Vitis vinifera L.) cultivars grown in the Lahore vineyards of Pakistan were selected. Real-time RT-PCR (TaqMan) assay was used before and after the regeneration of vine plants for the detection of GLRaVs. All nine cultivars were infected with two or more GLRaVs before apical meristem tip culture therapy. Apical meristem tip culture therapy was used as a mean to eliminate the GLRaVs from the optimum size (0.5 mm) of meristem tips. Out of 270 isolated meristems (30 each of nine cultivars), a total of 207 plants were regenerated with a 76.7% regeneration rate. The A7 cultivar demonstrated the highest regeneration rate (86.7%), while the A3 cultivar showed the lowest regeneration rate (66.7%). Screening after apical meristem tip culture therapy showed that the A1, A4, A5 & A8 cultivars demonstrated 100.0% elimination rate, while the A2, A3, A6, A7 & A9 cultivars demonstrated > 95.0% elimination rate, resulting in 97.5% overall elimination rate. The study concludes that apical meristem tip culture is an effective method for the elimination of GLRAVs infection from grapevine plants.

本研究旨在检测和消除市售葡萄品种中的葡萄卷叶相关病毒(GLRaV) ，以便产生适当的克隆用于认证。总共选择了在巴基斯坦拉合尔葡萄园种植的九个市售葡萄品种（Vitis vinifera L.）。在藤本植物再生前后使用实时 RT-PCR (TaqMan) 检测 GLRaV。在顶端分生组织尖端培养治疗之前，所有九个品种均感染了两种或多种 GLRaV。顶端分生组织尖端培养疗法被用作从分生组织尖端的最佳尺寸（0.5毫米）中消除GLRaV的方法。在 270 个分离的分生组织中（9 个品种各 30 个），总共再生了 207 株植物，再生率为 76.7%。A7品种的再生率最高（86.7%），而A3品种的再生率最低（66.7%）。顶端分生组织尖端培养治疗后的筛选显示，A1、A4、A5和A8品种的消除率为100.0%，而A2、A3、A6、A7和A9品种的消除率>95.0%，总体消除率为97.5% 。该研究得出的结论是，顶端分生组织尖端培养是消除葡萄植物中 GLRAV 感染的有效方法。