Resistance training (RT) can increase the heat shock response (HSR) in the elderly. As middle-aged subjects already suffer physiological declines related to aging, it is hypothesized that RT may increase the HSR in these people. To assess the effects of resistance training on heat shock response, intra and extracellular HSP70, oxidative stress, inflammation, body composition, and metabolism in middle-aged subjects. Sixteen volunteers (40 – 59 years) were allocated to two groups: the trained group (n = 7), which performed 12 weeks of RT; and the physically inactive—control group (n = 9), which did not perform any type of exercise. The RT program consisted of 9 whole-body exercises (using standard gym equipment) and functional exercises, carried out 3 times/week. Before and after the intervention, body composition, muscle mass, strength, functional capacity, and blood sample measurements (lipid profile, glucose, insulin, oxidative damage, TNF-α, the HSR, HSP70 expression in leukocytes, and HSP72 in plasma) were performed. The HSR analysis demonstrated that this response is maintained at normal levels in middle-aged people and that RT did not cause any improvement. Also, RT increases muscle mass, strength, and functional capacity. Despite no additional changes of RT on the antioxidant defenses (catalase, glutathione peroxidase, and reductase) or inflammation, lipid peroxidation was diminished by RT (group x time interaction, p = 0.009), indicating that other antioxidant defenses may be improved after RT. HSR is preserved in middle-aged subjects without metabolic complications. In addition, RT reduces lipid peroxidation and can retard muscle mass and strength loss related to the aging process.

阻力训练（RT）可以增加老年人的热休克反应（HSR）。由于中年受试者已经遭受与衰老相关的生理衰退，因此推测 RT 可能会增加这些人的 HSR。评估阻力训练对中年受试者的热休克反应、细胞内和细胞外 HSP70、氧化应激、炎症、身体成分和代谢的影响。16 名志愿者（40 – 59 岁）被分配到两组：训练组（n  = 7），进行 12 周的 RT；训练组（n = 7），进行 12 周的 RT。以及身体不活动的对照组（n  = 9），不进行任何类型的锻炼。RT计划包括9次全身练习（使用标准健身器材）和功能练习，每周进行3次。干预前后，对身体成分、肌肉质量、力量、功能能力和血样测量（血脂、血糖、胰岛素、氧化损伤、TNF-α、HSR、白细胞中 HSP70 表达和血浆中 HSP72）进行测量。执行。HSR 分析表明，这种反应在中年人中保持在正常水平，并且 RT 没有带来任何改善。此外，RT 还能增加肌肉质量、力量和功能能力。尽管 RT 对抗氧化防御（过氧化氢酶、谷胱甘肽过氧化物酶和还原酶）或炎症没有产生额外的变化，但 RT 减少了脂质过氧化（组 x 时间交互作用，p  = 0.009），表明 RT 后其他抗氧化防御可能会得到改善。HSR 在中年受试者中得以保留，没有代谢并发症。此外，放疗还可以减少脂质过氧化，并可以延缓与衰老过程相关的肌肉质量和力量损失。