Introduction: Aminoacyl tRNA synthetase complex interacting with multifunctional protein 2 (AIMP2) is a significant regulator of cell proliferation and apoptosis. Despite its abnormal expression in various tumor types, the specific functions and effects of AIMP2 on tumor immune cell infiltration, proliferation, and migration remain unclear. Materials and Methods: To assess AIMP2's role in tumor immunity, we conducted a pan-cancer multi-database analysis using the Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Cancer Cell Lines Encyclopedia (CCLE) datasets, examining expression levels, prognosis, tumor progression, and immune microenvironment. Additionally, we investigated AIMP2's impact on breast cancer (BRCA) proliferation and migration using cell counting kit 8 (CCK-8) assay, transwell assays, and western blot analysis. Result: Our findings revealed that AIMP2 was overexpressed in 24 tumor tissue types compared to normal tissue and was associated with four tumor stages. Survival analysis indicated that AIMP2 expression was strongly correlated with overall survival (OS) in certain cancer patients, with high AIMP2 expression linked to poorer prognosis in five cancer types. Conclusion: Finally, siRNA-mediated AIMP2 knockdown inhibited BRCA cell proliferation and migration in vitro. In conclusion, our pan-cancer analysis suggests that AIMP2 may play a crucial role in tumor immunity and could serve as a potential prognostic marker, particularly in BRCA.

中文翻译：

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泛癌分析确定 AIMP2 是乳腺癌的潜在生物标志物

简介：氨酰 tRNA 合成酶复合物与多功能蛋白 2 (AIMP2) 相互作用，是细胞增殖和凋亡的重要调节因子。尽管AIMP2在多种肿瘤类型中异常表达，但其对肿瘤免疫细胞浸润、增殖和迁移的具体功能和影响仍不清楚。材料和方法：为了评估 AIMP2 在肿瘤免疫中的作用，我们使用癌症基因组图谱 (TCGA)、基因型组织表达 (GTEx) 和癌细胞系百科全书 (CCLE) 数据集进行了泛癌多数据库分析，检查表达水平、预后、肿瘤进展和免疫微环境。此外，我们使用细胞计数试剂盒 8 (CCK-8) 测定、Transwell 测定和蛋白质印迹分析研究了 AIMP2 对乳腺癌 (BRCA) 增殖和迁移的影响。结果：我们的研究结果表明，与正常组织相比，AIMP2 在 24 种肿瘤组织类型中过度表达，并且与四个肿瘤阶段相关。生存分析表明，AIMP2 表达与某些癌症患者的总生存期 (OS) 密切相关，其中高 AIMP2 表达与五种癌症类型的较差预后相关。结论：最后，siRNA 介导的 AIMP2 敲低抑制了 BRCA 细胞的体外增殖和迁移。总之，我们的泛癌分析表明 AIMP2 可能在肿瘤免疫中发挥关键作用，并可作为潜在的预后标志物，特别是在 BRCA 中。