Background: LinB, as a Haloalkane dehalogenase, has good catalytic activity for many highly toxic and recalcitrant compounds, and can realize the elimination of chemical weapons HD in a green non-toxic mode. Objective: In order to display Haloalkane dehalogenase LinB on the surface of Bacillus subtilis spore Method: We have constituted the B. subtilis spore surface display system of halogenated alkanes dehalogenase LinB by gene recombination. Results: Data revealed that LinB can display on spore surface successfully. The hydrolyzing HD analogue 2-chloroethyl ethylsulfide (2-CEES) activity of displayed LinB spores was 4.30±0.09 U/mL, and its specific activity was 0.78±0.03U/mg. Meanwhile, LinB spores showed a stronger stress resistance activity on 2-CEES than free LinB. This study obtained B. subtilis spores of LinB (phingobium japonicum UT26) with enzyme activity that was not reported before. Conclusion: Spore surface display technology uses resistance spore as the carrier to guarantee LinB activity, enhances its stability, and reduces the production cost, thus expanding the range of its application.

中文翻译：

---

首次在枯草芽孢杆菌孢子表面展示卤代烷脱卤酶 LinB

背景：LinB作为卤代烷脱卤酶，对许多剧毒、难降解化合物具有良好的催化活性，可以实现绿色无毒模式消除化学武器HD。目的：将卤代烷脱卤酶LinB展示在枯草芽孢杆菌孢子表面。方法：通过基因重组构建了卤代烷脱卤酶LinB的枯草芽孢杆菌孢子表面展示系统。结果：数据显示LinB可以成功地在孢子表面展示。展示的LinB孢子的水解HD类似物2-氯乙基乙硫醚(2-CEES)活性为4.30±0.09 U/mL，其比活性为0.78±0.03U/mg。同时，LinB孢子对2-CEES表现出比游离LinB更强的抗逆活性。本研究获得的LinB（phingobium japonicum UT26）枯草芽孢杆菌孢子具有以前未报道过的酶活性。结论：孢子表面展示技术以抗性孢子为载体，保证了LinB活性，增强了其稳定性，降低了生产成本，从而扩大了其应用范围。