Background

Neuregulin (NRG) plays essential roles in the growth and differentiation of epithelial, neuronal and glial cells. Asthma is a chronic respiratory disease characterized by airway constriction and caused by allergens such as Der p 38.

Objective

This study is aimed to investigate the relationship between NRG-1 and asthma-like inflammation due to allergens.

Results

Following Der p 38 intranasal or intraperitoneal/intranasal (IP/IN) administration in mice, the expression levels of NRG-1 and its receptors increased in response, including ErbB2 and ErbB4. We further examined the roles of NRG-1 in the human BEAS-2B lung epithelial cell line. Der p 38 enhanced NRG-1 expression in the cells and NRG-1 secretion was also upregulated in BEAS-2B cell supernatants treated with Der p 38. NRG-1 alone did not increase the release of interleukin-6 (IL-6), IL-8, or monocyte chemoattractant protein-1 (MCP-1), but it inhibited cytokine secretion increased by Der p 38. Direct treatment with NRG-1 did not affect alteration of neutrophil apoptosis due to Der p 38, but BEAS-2B cell supernatants treated with Der p 38 and NRG-1 blocked the antiapoptotic effects of Der p 38-treated supernatants on neutrophils by activation of caspase 9 and caspase 3.

Conclusion

NRG-1 may exhibit anti-inflammatory effects in a Der p 38-induced allergenic environment, particularly neutrophilic inflammation. The present study on NRG may open up approach opportunities for understanding of pathogenesis and treatment of asthma.

中文翻译：

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Neuregulin-1 通过抑制细胞因子分泌来抑制 Der p 38 对中性粒细胞的抗凋亡作用

背景

神经调节蛋白 (NRG) 在上皮细胞、神经元和神经胶质细胞的生长和分化中发挥重要作用。哮喘是一种慢性呼吸道疾病，其特征是气道收缩，由 Der p 38 等过敏原引起。

客观的

本研究旨在探讨NRG-1与过敏原引起的哮喘样炎症之间的关系。

结果

在小鼠中鼻内或腹膜内/鼻内 (IP/IN) 施用 Der p 38 后，NRG-1 及其受体（包括 ErbB2 和 ErbB4）的表达水平相应增加。我们进一步研究了 NRG-1 在人 BEAS-2B 肺上皮细胞系中的作用。Der p 38 增强细胞中的 NRG-1 表达，并且在用 Der p 38 处理的 BEAS-2B 细胞上清液中，NRG-1 分泌也上调。单独的 NRG-1 不会增加白细胞介素 6 (IL-6) 的释放， IL-8，或单核细胞趋化蛋白-1 (MCP-1)，但它抑制 Der p 38 增加的细胞因子分泌。直接用 NRG-1 处理不影响 Der p 38 引起的中性粒细胞凋亡的改变，但 BEAS-2B用 Der p 38 和 NRG-1 处理的细胞上清液通过激活 caspase 9 和 caspase 3 阻断了 Der p 38 处理的上清液对中性粒细胞的抗凋亡作用。

结论

NRG-1 可能在 Der p 38 诱导的过敏环境中表现出抗炎作用，特别是中性粒细胞炎症。目前 NRG 的研究可能为了解哮喘的发病机制和治疗开辟新的途径。