Background

The skin microbiome, a diverse community of microorganisms, plays a crucial role in maintaining skin health. Among these microorganisms, the gram-positive bacterium Micrococcus luteus exhibits potential for promoting skin health. This study focuses on postbiotics derived from M. luteus YM-4, a strain isolated from human skin.

Objective

Our objective is to explore the beneficial effects of YM-4 culture filtrate on dermatological health, including enhancing barrier function, modulating immune response, and aiding recovery from environmental damage.

Methods

The effects of the YM-4 culture filtrate were tested on human keratinocytes and fibroblasts under various conditions using real-time PCR for gene expression analysis and fibroblast migration assays. A dehydration-simulated model was employed to prepare RNA-Seq samples from HaCaT cells treated with the YM-4 culture filtrate. Differentially expressed genes were identified and functionally classified through k-means clustering, gene ontology terms enrichment analyses, and protein-protein interactions mapping.

Results

The YM-4 culture filtrate enhanced the expression of genes involved in skin hydration, hyaluronic acid synthesis, barrier function, and cell proliferation. It also reduced inflammation markers in keratinocytes and fibroblasts under stress conditions. It mitigated UVB-induced collagen degradation while promoted collagen synthesis, suggesting anti-aging properties, and accelerated wound healing processes by promoting cell proliferation and migration. RNA sequencing analysis revealed that the YM-4 culture filtrate could reverse dehydration-induced transcriptional changes towards a state similar to untreated cells.

Conclusion

M. luteus YM-4 culture filtrate exhibits significant therapeutic potential for dermatological applications.

中文翻译：

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源自人体皮肤的藤黄微球菌的后生元对皮肤的益处：皮肤健康方面尚未开发的潜力

背景

皮肤微生物组是一个多样化的微生物群落，在维持皮肤健康方面发挥着至关重要的作用。在这些微生物中，革兰氏阳性细菌藤黄微球菌表现出促进皮肤健康的潜力。这项研究的重点是源自藤黄微球菌YM-4（一种从人体皮肤中分离出来的菌株）的后生元。

客观的

我们的目标是探索 YM-4 培养滤液对皮肤健康的有益影响，包括增强屏障功能、调节免疫反应和帮助从环境损害中恢复。

方法

使用实时 PCR 进行基因表达分析和成纤维细胞迁移测定，在各种条件下测试了 YM-4 培养滤液对人角质形成细胞和成纤维细胞的影响。采用脱水模拟模型从用 YM-4 培养滤液处理的 HaCaT 细胞中制备 RNA-Seq 样品。通过 k 均值聚类、基因本体术语富集分析和蛋白质-蛋白质相互作用作图来识别差异表达基因并进行功能分类。

结果

YM-4 培养滤液增强了涉及皮肤水合、透明质酸合成、屏障功能和细胞增殖的基因的表达。它还减少了应激条件下角质形成细胞和成纤维细胞中的炎症标志物。它可以减轻 UVB 诱导的胶原蛋白降解，同时促进胶原蛋白合成，表明具有抗衰老特性，并通过促进细胞增殖和迁移来加速伤口愈合过程。RNA测序分析表明，YM-4培养物滤液可以逆转脱水诱导的转录变化，使其达到与未处理细胞相似的状态。

结论

M. uteus YM-4 培养物滤液在皮肤病学应用中表现出显着的治疗潜力。