A new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075-0.02%). As a result, excellent linearity (R2 > 0.99) and precision (1.5-2.4% for repeatability and 0.7-2.2% for reproducibility) were achieved for the reference OPA solutions. Furthermore, reasonable concentration verification of OPA disinfection (0.2-0.6%) in healthcare institutes can be achieved using the developed fluorescent SIA due to its good sensitivity (0.111 V/%) and precision (1.0-2.3% for intermediate precision) around the minimum effective concentration (MEC) of 0.3% for Cidex-OPA disinfectant.

中文翻译：

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荧光连续进样分析法测定邻苯二甲醛，用于临床消毒剂剂量验证。

本研究报告了一种新的自动化通用分析方法，用于测定临床消毒剂邻苯二甲醛 (OPA)。所提出的顺序注射分析 (SIA) 基于 OPA 与甘氨酸/N-乙酰半胱氨酸 (NAC) 在中性介质 (pH = 7.0) 中的在线反应，形成高荧光异吲哚衍生物。研究了所有关键流量和反应变量，同时在线性检测范围 (0.0075-0.02%) 内进行验证。结果，参考 OPA 解决方案实现了出色的线性度（R2 > 0.99）和精度（重复性为 1.5-2.4%，再现性为 0.7-2.2%）。此外，利用所开发的荧光SIA，由于其良好的灵敏度（0.111 V/%）和精度（中间精度为1.0-2.3%），可以在医疗机构中实现OPA消毒的合理浓度验证（0.2-0.6%）。 Cidex-OPA 消毒剂的有效浓度 (MEC) 为 0.3%。