Lepidium ostleri S.L. Welsh & Goodrich (Ostler’s peppergrass) is an endemic plant species restricted to Ordovician limestone outcrops associated with the San Francisco Mountain Range in western Utah. Due to restricted population distribution and proximity to modern mining operations, L. ostleri is a species of conservation interest. This study focused on the development of a micropropagation protocol for propagating mature plants using plant tissue culture methods. Indirect shoot organogenesis was obtained from L. ostleri explants on Murashige and Skoog (MS) medium augmented with various concentrations of BAP (6-Benzylaminopurine), kinetin (N6-furfuryladenine), and IAA (indole-3-acetic acid). Plantlets supporting shoots grown in vitro were pulse treated with differing strengths of indole-3-butyric acid (IBA) and transferred to sterile soil. Following root induction, plantlets were acclimated to ambient conditions. The successful development of a micropropagation protocol supports management activities for L. ostleri and also contributes to in vitro propagation knowledge at the species, genus, and family levels.

Lepidium ostleri SL Welsh & Goodrich（奥斯特胡椒草）是一种特有植物物种，仅限于与犹他州西部旧金山山脉相关的奥陶纪石灰岩露头。由于种群分布有限且靠近现代采矿作业，L. ostleri是一种受保护的物种。这项研究的重点是开发使用植物组织培养方法繁殖成熟植物的微繁殖方案。间接芽器官发生是在 Murashige 和 Skoog (MS) 培养基上从L. ostleri外植体获得的，培养基中添加了不同浓度的 BAP（6-苄基氨基嘌呤）、激动素（N6-糠基腺嘌呤）和 IAA（吲哚-3-乙酸）。用不同强度的吲哚-3-丁酸（IBA）对支持体外生长的芽的小植株进行脉冲处理，并转移至无菌土壤中。根诱导后，使幼苗适应环境条件。微繁殖方案的成功开发支持了L. ostleri的管理活动，也有助于物种、属和科水平的体外繁殖知识。