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Development and characterization of 68 SNP markers in red sea bream, Pagrus major
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2023-11-21 , DOI: 10.1007/s12686-023-01337-z
Jeong Gyu Kim , Seung Hyun Yoo , Yong Bae Seo , Jong-Oh Kim , Gun-Do Kim

Red sea bream is a commercially valuable fish that is used as ingredients in the food industry. To prevent a decrease in fishing yield, farmed red sea bream is released. However, this may cause a decrease in the genetic diversity of wild populations, so systematic resource management is required. In this study, 68 single nucleotide polymorphism (SNP) markers were developed using a genotyping-by-sequencing (GBS) analysis. The minor allele frequency (MAF) ranged from 0.1802 to 0.5000, the observed heterozygosity (Ho) ranged from 0.0864 to 0.5000, expected heterozygosity (He) ranged from 0.2955 to 0.5000, and the polymorphism information content (PIC) ranged from 0.2518 to 0.3750. Of these SNPs, 25 loci significantly deviated from the Hardy–Weinberg equilibrium after a Bonferroni correction (p < 0.00074). These SNP markers will be useful for the further genetic analysis of the red sea bream population.



中文翻译:

真鲷 68 个 SNP 标记的开发和表征

红鲷鱼是一种具有商业价值的鱼类,用作食品工业的原料。为了防止捕捞量下降,养殖的红鲷鱼被放流。然而,这可能会导致野生种群遗传多样性的减少,因此需要系统的资源管理。在这项研究中,使用测序基因分型 (GBS) 分析开发了 68 个单核苷酸多态性 (SNP) 标记。次要等位基因频率(MAF)范围为0.1802至0.5000,观察杂合度(H o)范围为0.0864至0.5000,预期杂合度(He 范围为0.2955至0.5000,多态性信息内容(PIC)范围为0.2518至0.5000。 0.3750。在这些 SNP 中,25 个位点在 Bonferroni 校正后显着偏离 Hardy-Weinberg 平衡 ( p  < 0.00074)。这些SNP标记将有助于红鲷种群的进一步遗传分析。

更新日期:2023-11-22
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