In this study, the complete mitochondrial genomes of five noctuoid moth species, namely, Paectes cristatrix, Hemichloridia euprepia, Trigonodes hyppasia, Mythimna unipuncta, and Risoba obstructa, were newly sequenced and characterized. Based on our investigations, the five mitogenomes showed sizes of 15409, 15619, 15654, 15403, and 15233 bp, respectively, and encoded 37 genes (13 protein-coding, 2 ribosomal RNA, and 22 transfer RNA genes) and a control region. All protein-coding genes (PCGs) in these mitogenomes had typical ATN start codons except for cox1 and nad1. The non-canonical codon CGA was the starter in H. euprepia and M. unipuncta and TTG was the starter in T. hyppasia for cox1. The unusual codon GTG was the starter in R. obstructa for nad1. All tRNA genes showed distinctive putative cloverleaf structures except trnS1 (AGN), which lacked the dihydrouridine (DHU) stem. Bayesian inference (BI) and maximum likelihood (ML) analyses were employed based on 13 PCGs using 159 taxa among the five families of the superfamily Noctuoidea, and the results showed the monophyly of this superfamily with well-supported values. Phylogenetic analyses showed that the newly sequenced endemic species Hemichloridia euprepia clustered separately along with the subfamilies Cuculliinae and Acronictinae with fairly supported values.

在这项研究中，对五种夜蛾的完整线粒体基因组进行了新的测序和表征，即Paectes cristatrix、Hemicloridia euprepia、Trigonodes hyppasia、Mythimna unipuncta和Risobaobcessa。根据我们的研究，五个有丝分裂基因组的大小分别为 15409、15619、15654、15403 和 15233 bp，编码 37 个基因（13 个蛋白质编码基因、2 个核糖体 RNA 和 22 个转移 RNA 基因）和一个控制区。除cox1和nad1外，这些有丝分裂基因组中的所有蛋白质编码基因 (PCG) 均具有典型的 ATN 起始密码子。非规范密码子 CGA 是H. euprepia和M. unipuncta中的起始密码子，TTG 是T. hyppasia中cox1的起始密码子。不寻常的密码子 GTG 是R.obstructa中nad1的起始密码子。除trnS1 (AGN) 缺乏二氢尿苷 (DHU) 茎外，所有 tRNA 基因均显示出独特的推定三叶草结构。基于夜蛾总科五个科的 159 个分类单元的 13 个 PCG，采用贝叶斯推理 (BI) 和最大似然 (ML) 分析，结果表明该总科的单系性具有良好的支持值。系统发育分析表明，新测序的特有物种Hemichronidia euprepia与 Cuculliinae 和 Acronictinae 亚科分开聚类，具有相当支持的值。