Sponges (Porifera) harbor a diversity of microorganisms that contribute largely to the production a vast array of bioactive compounds. The microorganisms associated with sponge have an important impact on the chemical diversity of the natural products. Herein, our study focuses on an Aaptos suberitoides commonly found in Indonesia. The objective of this study was to investigate the profile of prokaryotic community and the presence of aaptamine metabolites in sponge Aaptos suberitoides. Sponges were collected from two site locations (Liberty Wreck and Drop Off) in Tulamben, Bali. The sponges were identified by barcoding DNA cytochrome oxidase subunit I (COI) gene. The profile of prokaryotic composition was investigated by amplifying the 16S rRNA gene using primers 515f and 806r to target the V4 region. The metabolites were analyzed using LC-MS, and dereplication was done to identify the aaptamines and its derivates. The barcoding DNA of the sponges confirmed the identity of samples as Aaptos suberitoides. The prokaryotic communities of samples A. suberitoides were enriched and dominated by taxa Proteobacteria, Chloroflexi, Actinobacteria, and Acidobacteria. The chemical analysis showed that all sponges produce aaptamine and isoaaptamine except A. suberitoides S2421 produce analog of aaptamines. This is the first report on the profile of prokaryotic community and the aaptamine of tropical marine sponges, A. suberitoides, from Tulamben, Bali.

海绵（Porifera）含有多种微生物，这些微生物在很大程度上有助于产生大量生物活性化合物。与海绵相关的微生物对天然产物的化学多样性具有重要影响。在此，我们的研究重点是印度尼西亚常见的Aaptos suberitoides 。本研究的目的是调查海绵Aaptos suberitoides 中原核生物群落的概况和适体胺代谢物的存在。海绵是从巴厘岛图兰奔的两个地点（Liberty Wreck 和 Drop Off）收集的。这些海绵通过 DNA 细胞色素氧化酶亚基 I (COI) 基因条形码进行鉴定。通过使用引物 515f 和 806r 靶向 V4 区域扩增 16S rRNA 基因来研究原核组成的概况。使用 LC-MS 分析代谢物，并进行去重复以鉴定适配胺及其衍生物。海绵的条形码 DNA 证实了样本的身份为Aaptos suberitoides。A. suberitoides样品的原核群落富集并以变形菌门、绿弯菌门、放线菌门和酸杆菌门为主。化学分析表明，除A. suberitoides S2421产生类似物外，所有海绵均产生适配胺和异适配胺。这是关于来自巴厘岛图兰本的热带海洋海绵A. suberitoides的原核生物群落和适体胺概况的第一份报告。