Stem rust caused by the fungus Puccinia graminis Pers. is a dangerous disease of wheat that occurs in all regions of its cultivation. New highly virulent races causing severe yield losses have appeared in recent decades. Sr33 introgressed from Aegilops tauschii is one of the genes conferring resistance against most races of stem rust, including Ug99. To develop winter common wheat lines with the gene Sr33 and evaluate a possible effect of the gene on yield traits, the authors made a cross between the spring line DH31 carrying the Sr33 gene and the winter cultivar Myrkhad followed by marker-assisted selection of winter genotypes with Sr33 starting from F₃. To identify the Sr33 gene, PCR with the gene-specific marker Sr33A was used. Alleles at the storage protein loci of the parental forms were identified using acid polyacrylamide gel electrophoresis and SDS-electrophoresis as well as the molecular marker MAR for Glu-Blal. As a result of marker-assisted selection, winter F₅ lines with the Sr33 gene were developed from the cross DH31 × Myrkhad. The yield traits of F₅ spikes of families derived from single F₃ spikes with and without Sr33 were analyzed considering that the line DH31 has a specific allele at Gli-D1 from Ae. tauschii, dark glumes, and high molecular weight glutenin subunit alleles associated with high dough strength, in particular, Glu-Blal. Comparison of means of yield traits of spikes from families with Sr33 and without it did not reveal significant differences between these two groups. Thus, winter F₅ lines with the Sr33 gene from the cross DH31 × Myrkhad may be used in the breeding practice to develop cultivars with high bread-making qualities of flour and stem rust resistance.

由真菌禾本科柄锈菌引起的茎锈病。是一种危险的小麦病害，发生在所有小麦种植地区。近几十年来，出现了导致严重产量损失的新的高毒力品种。从节节山羊草渗入的Sr33是赋予对大多数茎锈病（包括 Ug99）抗性的基因之一。为了开发带有Sr33基因的冬季普通小麦品系并评估该基因对产量性状的可能影响，作者在携带Sr33基因的春季品系 DH31 和冬季品种 Myrkhad之间进行杂交，然后对冬季基因型进行标记辅助选择Sr33从F ₃开始。为了鉴定Sr33基因，使用了带有基因特异性标记Sr33A的 PCR 。使用酸性聚丙烯酰胺凝胶电泳和SDS电泳以及Glu-Blal的分子标记MAR鉴定亲本形式的储存蛋白基因座处的等位基因。作为标记辅助选择的结果，带有Sr33基因的冬季F ₅品系是从DH31×Myrkhad杂交中培育出来的。考虑到系DH31在来自Ae的Gli-D1处具有特定等位基因，分析了源自具有和不具有Sr33的单个F ₃穗的家族的F ₅穗的产量性状。节节花、深色颖片和高分子量麦谷蛋白亚基等位基因与高面团强度相关，特别是Glu-Blal。对含有Sr33和不含 Sr33的家系的穗产量性状平均值进行比较，并未发现这两组之间存在显着差异。因此，来自DH31×Myrkhad杂交的带有Sr33基因的冬季F ₅系可用于育种实践中，以开发具有高面粉制作品质和茎锈病抗性的品种。